Northern blotting analysis

TY - CHAP

T1 - Northern blotting analysis

AU - Josefsen, Knud

AU - Nielsen, Henrik

PY - 2011/1/1

Y1 - 2011/1/1

N2 - Northern blotting analysis is a classical method for analysis of the size and steady-state level of a specific RNA in a complex sample. In short, the RNA is size-fractionated by gel electrophoresis and transferred by blotting onto a membrane to which the RNA is covalently bound. Then, the membrane is analysed by hybridization to one or more specific probes that are labelled for subsequent detection. Northern blotting is relatively simple to perform, inexpensive, and not plagued by artefacts. Recent developments of hybridization membranes and buffers have resulted in increased sensitivity closing the gap to the more laborious nuclease protection experiments.

AB - Northern blotting analysis is a classical method for analysis of the size and steady-state level of a specific RNA in a complex sample. In short, the RNA is size-fractionated by gel electrophoresis and transferred by blotting onto a membrane to which the RNA is covalently bound. Then, the membrane is analysed by hybridization to one or more specific probes that are labelled for subsequent detection. Northern blotting is relatively simple to perform, inexpensive, and not plagued by artefacts. Recent developments of hybridization membranes and buffers have resulted in increased sensitivity closing the gap to the more laborious nuclease protection experiments.

KW - Blotting, Northern

KW - Electrophoresis, Agar Gel

KW - Electrophoresis, Polyacrylamide Gel

KW - Formaldehyde

KW - Glyoxal

KW - Membranes, Artificial

KW - RNA

U2 - 10.1007/978-1-59745-248-9_7

DO - 10.1007/978-1-59745-248-9_7

M3 - Book chapter

C2 - 21125485

VL - 703

SP - 87

EP - 105

BT - Methods in Molecular Biology

PB - Springer Science+Business Media

ER -