Noninvasive optical inhibition with a red-shifted microbial rhodopsin

Amy S Chuong; Mitra L Miri; Volker Busskamp; Gillian A C Matthews; Leah C Acker; Andreas T Sørensen; Andrew Young; Nathan C Klapoetke; Mike A Henninger; Suhasa B Kodandaramaiah; Masaaki Ogawa; Shreshtha B Ramanlal; Rachel C Bandler; Brian D Allen; Craig R Forest; Brian Y Chow; Xue Han; Yingxi Lin; Kay M Tye; Botond Roska; Jessica A Cardin; Edward S Boyden

doi:10.1038/nn.3752

Noninvasive optical inhibition with a red-shifted microbial rhodopsin

Amy S Chuong, Mitra L Miri, Volker Busskamp, Gillian A C Matthews, Leah C Acker, Andreas T Sørensen, Andrew Young, Nathan C Klapoetke, Mike A Henninger, Suhasa B Kodandaramaiah, Masaaki Ogawa, Shreshtha B Ramanlal, Rachel C Bandler, Brian D Allen, Craig R Forest, Brian Y Chow, Xue Han, Yingxi Lin, Kay M Tye, Botond RoskaJessica A Cardin, Edward S Boyden

281 Citations (Scopus)

Abstract

Optogenetic inhibition of the electrical activity of neurons enables the causal assessment of their contributions to brain functions. Red light penetrates deeper into tissue than other visible wavelengths. We present a red-shifted cruxhalorhodopsin, Jaws, derived from Haloarcula (Halobacterium) salinarum (strain Shark) and engineered to result in red light-induced photocurrents three times those of earlier silencers. Jaws exhibits robust inhibition of sensory-evoked neural activity in the cortex and results in strong light responses when used in retinas of retinitis pigmentosa model mice. We also demonstrate that Jaws can noninvasively mediate transcranial optical inhibition of neurons deep in the brains of awake mice. The noninvasive optogenetic inhibition opened up by Jaws enables a variety of important neuroscience experiments and offers a powerful general-use chloride pump for basic and applied neuroscience.

Original language	English
Journal	Nature Neuroscience
Volume	17
Issue number	8
Pages (from-to)	1123-1129
Number of pages	7
ISSN	1097-6256
DOIs	https://doi.org/10.1038/nn.3752
Publication status	Published - Aug 2014
Externally published	Yes

Keywords

Animals
Brain Chemistry
Halobacterium salinarum
Halorhodopsins
Mice
Molecular Sequence Data
Neural Inhibition
Neurons
Optogenetics
Retina
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Technical Report

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Chuong, A. S., Miri, M. L., Busskamp, V., Matthews, G. A. C., Acker, L. C., Sørensen, A. T., Young, A., Klapoetke, N. C., Henninger, M. A., Kodandaramaiah, S. B., Ogawa, M., Ramanlal, S. B., Bandler, R. C., Allen, B. D., Forest, C. R., Chow, B. Y., Han, X., Lin, Y., Tye, K. M., ... Boyden, E. S. (2014). Noninvasive optical inhibition with a red-shifted microbial rhodopsin. Nature Neuroscience, 17(8), 1123-1129. https://doi.org/10.1038/nn.3752

Chuong, AS, Miri, ML, Busskamp, V, Matthews, GAC, Acker, LC, Sørensen, AT, Young, A, Klapoetke, NC, Henninger, MA, Kodandaramaiah, SB, Ogawa, M, Ramanlal, SB, Bandler, RC, Allen, BD, Forest, CR, Chow, BY, Han, X, Lin, Y, Tye, KM, Roska, B, Cardin, JA & Boyden, ES 2014, 'Noninvasive optical inhibition with a red-shifted microbial rhodopsin', Nature Neuroscience, vol. 17, no. 8, pp. 1123-1129. https://doi.org/10.1038/nn.3752

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abstract = "Optogenetic inhibition of the electrical activity of neurons enables the causal assessment of their contributions to brain functions. Red light penetrates deeper into tissue than other visible wavelengths. We present a red-shifted cruxhalorhodopsin, Jaws, derived from Haloarcula (Halobacterium) salinarum (strain Shark) and engineered to result in red light-induced photocurrents three times those of earlier silencers. Jaws exhibits robust inhibition of sensory-evoked neural activity in the cortex and results in strong light responses when used in retinas of retinitis pigmentosa model mice. We also demonstrate that Jaws can noninvasively mediate transcranial optical inhibition of neurons deep in the brains of awake mice. The noninvasive optogenetic inhibition opened up by Jaws enables a variety of important neuroscience experiments and offers a powerful general-use chloride pump for basic and applied neuroscience.",

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AU - Sørensen, Andreas T

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AU - Bandler, Rachel C

AU - Allen, Brian D

AU - Forest, Craig R

AU - Chow, Brian Y

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JO - Nature Neuroscience

JF - Nature Neuroscience

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ER -

Noninvasive optical inhibition with a red-shifted microbial rhodopsin

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Keywords

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