Noninvasive optical inhibition with a red-shifted microbial rhodopsin

Amy S Chuong; Mitra L Miri; Volker Busskamp; Gillian A C Matthews; Leah C Acker; Andreas T Sørensen; Andrew Young; Nathan C Klapoetke; Mike A Henninger; Suhasa B Kodandaramaiah; Masaaki Ogawa; Shreshtha B Ramanlal; Rachel C Bandler; Brian D Allen; Craig R Forest; Brian Y Chow; Xue Han; Yingxi Lin; Kay M Tye; Botond Roska; Jessica A Cardin; Edward S Boyden

doi:10.1038/nn.3752

Noninvasive optical inhibition with a red-shifted microbial rhodopsin

Amy S Chuong, Mitra L Miri, Volker Busskamp, Gillian A C Matthews, Leah C Acker, Andreas T Sørensen, Andrew Young, Nathan C Klapoetke, Mike A Henninger, Suhasa B Kodandaramaiah, Masaaki Ogawa, Shreshtha B Ramanlal, Rachel C Bandler, Brian D Allen, Craig R Forest, Brian Y Chow, Xue Han, Yingxi Lin, Kay M Tye, Botond RoskaJessica A Cardin, Edward S Boyden

281 Citationer (Scopus)

Abstract

Optogenetic inhibition of the electrical activity of neurons enables the causal assessment of their contributions to brain functions. Red light penetrates deeper into tissue than other visible wavelengths. We present a red-shifted cruxhalorhodopsin, Jaws, derived from Haloarcula (Halobacterium) salinarum (strain Shark) and engineered to result in red light-induced photocurrents three times those of earlier silencers. Jaws exhibits robust inhibition of sensory-evoked neural activity in the cortex and results in strong light responses when used in retinas of retinitis pigmentosa model mice. We also demonstrate that Jaws can noninvasively mediate transcranial optical inhibition of neurons deep in the brains of awake mice. The noninvasive optogenetic inhibition opened up by Jaws enables a variety of important neuroscience experiments and offers a powerful general-use chloride pump for basic and applied neuroscience.

Originalsprog	Engelsk
Tidsskrift	Nature Neuroscience
Vol/bind	17
Udgave nummer	8
Sider (fra-til)	1123-1129
Antal sider	7
ISSN	1097-6256
DOI	https://doi.org/10.1038/nn.3752
Status	Udgivet - aug. 2014
Udgivet eksternt	Ja

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10.1038/nn.3752

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Chuong, A. S., Miri, M. L., Busskamp, V., Matthews, G. A. C., Acker, L. C., Sørensen, A. T., Young, A., Klapoetke, N. C., Henninger, M. A., Kodandaramaiah, S. B., Ogawa, M., Ramanlal, S. B., Bandler, R. C., Allen, B. D., Forest, C. R., Chow, B. Y., Han, X., Lin, Y., Tye, K. M., ... Boyden, E. S. (2014). Noninvasive optical inhibition with a red-shifted microbial rhodopsin. Nature Neuroscience, 17(8), 1123-1129. https://doi.org/10.1038/nn.3752

Chuong, AS, Miri, ML, Busskamp, V, Matthews, GAC, Acker, LC, Sørensen, AT, Young, A, Klapoetke, NC, Henninger, MA, Kodandaramaiah, SB, Ogawa, M, Ramanlal, SB, Bandler, RC, Allen, BD, Forest, CR, Chow, BY, Han, X, Lin, Y, Tye, KM, Roska, B, Cardin, JA & Boyden, ES 2014, 'Noninvasive optical inhibition with a red-shifted microbial rhodopsin', Nature Neuroscience, bind 17, nr. 8, s. 1123-1129. https://doi.org/10.1038/nn.3752

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abstract = "Optogenetic inhibition of the electrical activity of neurons enables the causal assessment of their contributions to brain functions. Red light penetrates deeper into tissue than other visible wavelengths. We present a red-shifted cruxhalorhodopsin, Jaws, derived from Haloarcula (Halobacterium) salinarum (strain Shark) and engineered to result in red light-induced photocurrents three times those of earlier silencers. Jaws exhibits robust inhibition of sensory-evoked neural activity in the cortex and results in strong light responses when used in retinas of retinitis pigmentosa model mice. We also demonstrate that Jaws can noninvasively mediate transcranial optical inhibition of neurons deep in the brains of awake mice. The noninvasive optogenetic inhibition opened up by Jaws enables a variety of important neuroscience experiments and offers a powerful general-use chloride pump for basic and applied neuroscience.",

keywords = "Animals, Brain Chemistry, Halobacterium salinarum, Halorhodopsins, Mice, Molecular Sequence Data, Neural Inhibition, Neurons, Optogenetics, Retina, Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S., Technical Report",

author = "Chuong, {Amy S} and Miri, {Mitra L} and Volker Busskamp and Matthews, {Gillian A C} and Acker, {Leah C} and S{\o}rensen, {Andreas T} and Andrew Young and Klapoetke, {Nathan C} and Henninger, {Mike A} and Kodandaramaiah, {Suhasa B} and Masaaki Ogawa and Ramanlal, {Shreshtha B} and Bandler, {Rachel C} and Allen, {Brian D} and Forest, {Craig R} and Chow, {Brian Y} and Xue Han and Yingxi Lin and Tye, {Kay M} and Botond Roska and Cardin, {Jessica A} and Boyden, {Edward S}",

year = "2014",

month = aug,

doi = "10.1038/nn.3752",

language = "English",

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journal = "Nature Neuroscience",

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AU - Chuong, Amy S

AU - Miri, Mitra L

AU - Busskamp, Volker

AU - Matthews, Gillian A C

AU - Acker, Leah C

AU - Sørensen, Andreas T

AU - Young, Andrew

AU - Klapoetke, Nathan C

AU - Henninger, Mike A

AU - Kodandaramaiah, Suhasa B

AU - Ogawa, Masaaki

AU - Ramanlal, Shreshtha B

AU - Bandler, Rachel C

AU - Allen, Brian D

AU - Forest, Craig R

AU - Chow, Brian Y

AU - Han, Xue

AU - Lin, Yingxi

AU - Tye, Kay M

AU - Roska, Botond

AU - Cardin, Jessica A

AU - Boyden, Edward S

PY - 2014/8

Y1 - 2014/8

N2 - Optogenetic inhibition of the electrical activity of neurons enables the causal assessment of their contributions to brain functions. Red light penetrates deeper into tissue than other visible wavelengths. We present a red-shifted cruxhalorhodopsin, Jaws, derived from Haloarcula (Halobacterium) salinarum (strain Shark) and engineered to result in red light-induced photocurrents three times those of earlier silencers. Jaws exhibits robust inhibition of sensory-evoked neural activity in the cortex and results in strong light responses when used in retinas of retinitis pigmentosa model mice. We also demonstrate that Jaws can noninvasively mediate transcranial optical inhibition of neurons deep in the brains of awake mice. The noninvasive optogenetic inhibition opened up by Jaws enables a variety of important neuroscience experiments and offers a powerful general-use chloride pump for basic and applied neuroscience.

AB - Optogenetic inhibition of the electrical activity of neurons enables the causal assessment of their contributions to brain functions. Red light penetrates deeper into tissue than other visible wavelengths. We present a red-shifted cruxhalorhodopsin, Jaws, derived from Haloarcula (Halobacterium) salinarum (strain Shark) and engineered to result in red light-induced photocurrents three times those of earlier silencers. Jaws exhibits robust inhibition of sensory-evoked neural activity in the cortex and results in strong light responses when used in retinas of retinitis pigmentosa model mice. We also demonstrate that Jaws can noninvasively mediate transcranial optical inhibition of neurons deep in the brains of awake mice. The noninvasive optogenetic inhibition opened up by Jaws enables a variety of important neuroscience experiments and offers a powerful general-use chloride pump for basic and applied neuroscience.

KW - Animals

KW - Brain Chemistry

KW - Halobacterium salinarum

KW - Halorhodopsins

KW - Mice

KW - Molecular Sequence Data

KW - Neural Inhibition

KW - Neurons

KW - Optogenetics

KW - Retina

KW - Journal Article

KW - Research Support, N.I.H., Extramural

KW - Research Support, Non-U.S. Gov't

KW - Research Support, U.S. Gov't, Non-P.H.S.

KW - Technical Report

U2 - 10.1038/nn.3752

DO - 10.1038/nn.3752

M3 - Journal article

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EP - 1129

JO - Nature Neuroscience

JF - Nature Neuroscience

IS - 8

ER -

Noninvasive optical inhibition with a red-shifted microbial rhodopsin

Abstract

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