TY - JOUR
T1 - Non-MHC genes influence virus clearance through regulation of the antiviral T-cell response: correlation between virus clearance and Tc and Td activity in segregating backcross progeny
AU - Christensen, Jan Pravsgaard
AU - Marker, O
AU - Thomsen, Allan Randrup
N1 - Keywords: Animals; Crosses, Genetic; Cytotoxicity, Immunologic; Female; Hypersensitivity, Delayed; Lymphocytic Choriomeningitis; Lymphocytic choriomeningitis virus; Male; Mice; Mice, Inbred BALB C; Mice, Inbred DBA; Sex Factors; T-Lymphocytes; X Chromosome
PY - 1994
Y1 - 1994
N2 - To determine the mechanism by which non-MHC genes control the rate of virus clearance in mice infected with lymphocytic choriomeningitis virus, a segregating backcross population was studied. Thirty BC1 animals were infected with virus, and virus-specific delayed-type hypersensitivity (DTH) was followed by measurement of footpad swelling. Ten days after virus inoculation, the animals were sacrificed and spleen virus titer together with splenic Tc activity was measured. With regard to all three parameters a continuous distribution was observed in this backcross population. However, using cutoff values based on parental and F1 animals tested in parallel, 11/30 animals were assigned Tc responders, 23/30 DTH responders and 10/30 cleared virus with maximal efficiency. Comparison of responder status with regard to the different parameters revealed a strong correlation between Tc responsiveness and the ability to clear virus. Amongst Tc low responders a correlation between DTH reactivity and virus clearance was observed. Taken together, these results indicate that non-MHC genes affect virus clearance through regulation of the antiviral T-cell response, especially the virus-specific Tc response. However, also virus-specific DTH reactivity seems important, particularly when Tc activity is low.
AB - To determine the mechanism by which non-MHC genes control the rate of virus clearance in mice infected with lymphocytic choriomeningitis virus, a segregating backcross population was studied. Thirty BC1 animals were infected with virus, and virus-specific delayed-type hypersensitivity (DTH) was followed by measurement of footpad swelling. Ten days after virus inoculation, the animals were sacrificed and spleen virus titer together with splenic Tc activity was measured. With regard to all three parameters a continuous distribution was observed in this backcross population. However, using cutoff values based on parental and F1 animals tested in parallel, 11/30 animals were assigned Tc responders, 23/30 DTH responders and 10/30 cleared virus with maximal efficiency. Comparison of responder status with regard to the different parameters revealed a strong correlation between Tc responsiveness and the ability to clear virus. Amongst Tc low responders a correlation between DTH reactivity and virus clearance was observed. Taken together, these results indicate that non-MHC genes affect virus clearance through regulation of the antiviral T-cell response, especially the virus-specific Tc response. However, also virus-specific DTH reactivity seems important, particularly when Tc activity is low.
M3 - Journal article
C2 - 8031572
SN - 0254-9670
VL - 11
SP - 33
EP - 44
JO - Experimental and Clinical Immunogenetics
JF - Experimental and Clinical Immunogenetics
IS - 1
ER -