Non-invasive in-cell determination of free cytosolic [NAD+]/[NADH] ratios using hyperpolarized glucose show large variations in metabolic phenotypes

Caspar Elo Christensen; Magnus Karlsson; Jakob R. Winther; Pernille Rose Jensen; Mathilde H. Lerche

doi:10.1074/jbc.M113.498626

Non-invasive in-cell determination of free cytosolic [NAD+]/[NADH] ratios using hyperpolarized glucose show large variations in metabolic phenotypes

Caspar Elo Christensen, Magnus Karlsson, Jakob R. Winther, Pernille Rose Jensen, Mathilde H. Lerche

Biomolecular Sciences

77 Citations (Scopus)

Abstract

Background: Free cytosolic [NAD⁺]/[NADH] ratio maintains cellular redox homeostasis and is a cellular metabolic readout. Results: Pyruvate/lactate ratios show distinct metabolic phenotypes and are used to derive free cytosolic [NAD⁺]/[NADH] ratios. Conclusion: Determination of free cytosolic [NAD⁺]/[NADH] ratios using hyperpolarized glucose is applicable to a wide selection of cell types. Significance: This metabolic phenotyping may be a crucial tool to understand pathologies, and to diagnose and measure effects of therapies.

Original language	English
Journal	The Journal of Biological Chemistry
Volume	289
Issue number	4
Pages (from-to)	2344-2352
Number of pages	9
ISSN	0021-9258
DOIs	https://doi.org/10.1074/jbc.M113.498626
Publication status	Published - 24 Jan 2014

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10.1074/jbc.M113.498626

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Non-invasive in-cell determination of free cytosolic [NAD+]/[NADH] ratios using hyperpolarized glucose show large variations in metabolic phenotypes. / Christensen, Caspar Elo; Karlsson, Magnus; Winther, Jakob R. et al.

In: The Journal of Biological Chemistry, Vol. 289, No. 4, 24.01.2014, p. 2344-2352.

Research output: Contribution to journal › Journal article › Research › peer-review

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abstract = "Background: Free cytosolic [NAD+]/[NADH] ratio maintains cellular redox homeostasis and is a cellular metabolic readout. Results: Pyruvate/lactate ratios show distinct metabolic phenotypes and are used to derive free cytosolic [NAD+]/[NADH] ratios. Conclusion: Determination of free cytosolic [NAD+]/[NADH] ratios using hyperpolarized glucose is applicable to a wide selection of cell types. Significance: This metabolic phenotyping may be a crucial tool to understand pathologies, and to diagnose and measure effects of therapies.",

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AU - Jensen, Pernille Rose

AU - Lerche, Mathilde H.

PY - 2014/1/24

Y1 - 2014/1/24

N2 - Background: Free cytosolic [NAD+]/[NADH] ratio maintains cellular redox homeostasis and is a cellular metabolic readout. Results: Pyruvate/lactate ratios show distinct metabolic phenotypes and are used to derive free cytosolic [NAD+]/[NADH] ratios. Conclusion: Determination of free cytosolic [NAD+]/[NADH] ratios using hyperpolarized glucose is applicable to a wide selection of cell types. Significance: This metabolic phenotyping may be a crucial tool to understand pathologies, and to diagnose and measure effects of therapies.

AB - Background: Free cytosolic [NAD+]/[NADH] ratio maintains cellular redox homeostasis and is a cellular metabolic readout. Results: Pyruvate/lactate ratios show distinct metabolic phenotypes and are used to derive free cytosolic [NAD+]/[NADH] ratios. Conclusion: Determination of free cytosolic [NAD+]/[NADH] ratios using hyperpolarized glucose is applicable to a wide selection of cell types. Significance: This metabolic phenotyping may be a crucial tool to understand pathologies, and to diagnose and measure effects of therapies.

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Non-invasive in-cell determination of free cytosolic [NAD+]/[NADH] ratios using hyperpolarized glucose show large variations in metabolic phenotypes

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