Non-invasive in-cell determination of free cytosolic [NAD+]/[NADH] ratios using hyperpolarized glucose show large variations in metabolic phenotypes

TY - JOUR

T1 - Non-invasive in-cell determination of free cytosolic [NAD+]/[NADH] ratios using hyperpolarized glucose show large variations in metabolic phenotypes

AU - Christensen, Caspar Elo

AU - Karlsson, Magnus

AU - Winther, Jakob R.

AU - Jensen, Pernille Rose

AU - Lerche, Mathilde H.

PY - 2014/1/24

Y1 - 2014/1/24

N2 - Background: Free cytosolic [NAD+]/[NADH] ratio maintains cellular redox homeostasis and is a cellular metabolic readout. Results: Pyruvate/lactate ratios show distinct metabolic phenotypes and are used to derive free cytosolic [NAD+]/[NADH] ratios. Conclusion: Determination of free cytosolic [NAD+]/[NADH] ratios using hyperpolarized glucose is applicable to a wide selection of cell types. Significance: This metabolic phenotyping may be a crucial tool to understand pathologies, and to diagnose and measure effects of therapies.

AB - Background: Free cytosolic [NAD+]/[NADH] ratio maintains cellular redox homeostasis and is a cellular metabolic readout. Results: Pyruvate/lactate ratios show distinct metabolic phenotypes and are used to derive free cytosolic [NAD+]/[NADH] ratios. Conclusion: Determination of free cytosolic [NAD+]/[NADH] ratios using hyperpolarized glucose is applicable to a wide selection of cell types. Significance: This metabolic phenotyping may be a crucial tool to understand pathologies, and to diagnose and measure effects of therapies.

U2 - 10.1074/jbc.M113.498626

DO - 10.1074/jbc.M113.498626

M3 - Journal article

C2 - 24302737

SN - 0021-9258

VL - 289

SP - 2344

EP - 2352

JO - The Journal of Biological Chemistry

JF - The Journal of Biological Chemistry

IS - 4

ER -