New Nordic Diet induced weight loss is accompanied by changes in metabolism and AMPK signalling in adipose tissue

Andreas Mæchel Fritzen; Annemarie Lundsgaard; Andreas Børsting Jordy; Sanne Kellebjerg Poulsen; Steen Stender; Henriette Pilegaard; Arne Astrup; Thomas Meinert Larsen; Jørgen Wojtaszewski; Erik Richter; Bente Kiens

doi:10.1210/jc.2015-2079

New Nordic Diet induced weight loss is accompanied by changes in metabolism and AMPK signalling in adipose tissue

Andreas Mæchel Fritzen, Annemarie Lundsgaard, Andreas Børsting Jordy, Sanne Kellebjerg Poulsen, Steen Stender, Henriette Pilegaard, Arne Astrup, Thomas Meinert Larsen, Jørgen Wojtaszewski, Erik Richter, Bente Kiens

28 Citations (Scopus)

Abstract

Context: The molecular mechanisms behind diet-induced metabolic improvements remain to be studied. Objective: This study sought to investigate whether expression of proteins in skeletal muscle or adipose tissue could explain improvements in glucose and lipid homeostasis after weight loss. Design: Volunteers consumed a New Nordic Diet (NND) or an Average Danish Diet for 26 weeks in a controlled, free-living setting. Subjects: Sixty four moderately obese women and men (44 ± 2 y; body mass index, 31 ± 1 kg/m²). Intervention: Fasting blood samples and biopsies from the vastus lateralis muscle and subcutaneous abdominal adipose tissue (SCAT) were obtained at week 0 and 26. Outcome: Gene and protein expressions were analyzed by real-time PCR and Western blotting. Results: Improved homeostasis homeostatic model of assessment-insulin resistance index and lowered plasma triacylglycerol concentration afterNNDcoincided with molecular adaptations in SCAT but not in skeletal muscle. NND induced greater reduction in fat mass than ADD (-6 ± 1 kg and -2 ± 1 kg; P < .01). In SCAT this was associated with increased AMPK and acetyl-CoA carboxylase phosphorylation (P < .05). Concomitantly, NND induced up-regulation of Akt2 and Akt substrate of 160 kDa (P < .05) as well as fatty acid transport protein 4 and membrane associated fatty acid binding protein (P < .05). Indices of increased oxidative capacity were observed, as carnitine palmitoyl transferase 1 mRNA (P = .08) as well as citrate synthase (P = .1) and cytochrome c (P = .05) protein tended to increase. Conclusion: NND-induced metabolic improvements were accompanied by increased AMPK signaling in SCAT, suggesting a role ofAMPKin these adaptations. The concomitant up-regulation of key glucose and lipid-handling proteins suggests an improved metabolic capacity in adipose tissue after weight loss.

Original language	English
Journal	Journal of Clinical Endocrinology and Metabolism
Volume	100
Issue number	9
Pages (from-to)	3509-3519
Number of pages	11
ISSN	0021-972X
DOIs	https://doi.org/10.1210/jc.2015-2079
Publication status	Published - 1 Sept 2015

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Fritzen, A. M., Lundsgaard, A., Jordy, A. B., Poulsen, S. K., Stender, S., Pilegaard, H., Astrup, A., Larsen, T. M., Wojtaszewski, J., Richter, E., & Kiens, B. (2015). New Nordic Diet induced weight loss is accompanied by changes in metabolism and AMPK signalling in adipose tissue. Journal of Clinical Endocrinology and Metabolism, 100(9), 3509-3519. https://doi.org/10.1210/jc.2015-2079

New Nordic Diet induced weight loss is accompanied by changes in metabolism and AMPK signalling in adipose tissue. / Fritzen, Andreas Mæchel ; Lundsgaard, Annemarie; Jordy, Andreas Børsting et al.
In: Journal of Clinical Endocrinology and Metabolism, Vol. 100, No. 9, 01.09.2015, p. 3509-3519.

Research output: Contribution to journal › Journal article › Research › peer-review

Fritzen, AM , Lundsgaard, A, Jordy, AB, Poulsen, SK, Stender, S, Pilegaard, H, Astrup, A, Larsen, TM, Wojtaszewski, J , Richter, E & Kiens, B 2015, 'New Nordic Diet induced weight loss is accompanied by changes in metabolism and AMPK signalling in adipose tissue', Journal of Clinical Endocrinology and Metabolism, vol. 100, no. 9, pp. 3509-3519. https://doi.org/10.1210/jc.2015-2079

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title = "New Nordic Diet induced weight loss is accompanied by changes in metabolism and AMPK signalling in adipose tissue",

abstract = "Context: The molecular mechanisms behind diet-induced metabolic improvements remain to be studied. Objective: This study sought to investigate whether expression of proteins in skeletal muscle or adipose tissue could explain improvements in glucose and lipid homeostasis after weight loss. Design: Volunteers consumed a New Nordic Diet (NND) or an Average Danish Diet for 26 weeks in a controlled, free-living setting. Subjects: Sixty four moderately obese women and men (44 ± 2 y; body mass index, 31 ± 1 kg/m2). Intervention: Fasting blood samples and biopsies from the vastus lateralis muscle and subcutaneous abdominal adipose tissue (SCAT) were obtained at week 0 and 26. Outcome: Gene and protein expressions were analyzed by real-time PCR and Western blotting. Results: Improved homeostasis homeostatic model of assessment-insulin resistance index and lowered plasma triacylglycerol concentration afterNNDcoincided with molecular adaptations in SCAT but not in skeletal muscle. NND induced greater reduction in fat mass than ADD (-6 ± 1 kg and -2 ± 1 kg; P < .01). In SCAT this was associated with increased AMPK and acetyl-CoA carboxylase phosphorylation (P < .05). Concomitantly, NND induced up-regulation of Akt2 and Akt substrate of 160 kDa (P < .05) as well as fatty acid transport protein 4 and membrane associated fatty acid binding protein (P < .05). Indices of increased oxidative capacity were observed, as carnitine palmitoyl transferase 1 mRNA (P = .08) as well as citrate synthase (P = .1) and cytochrome c (P = .05) protein tended to increase. Conclusion: NND-induced metabolic improvements were accompanied by increased AMPK signaling in SCAT, suggesting a role ofAMPKin these adaptations. The concomitant up-regulation of key glucose and lipid-handling proteins suggests an improved metabolic capacity in adipose tissue after weight loss.",

author = "Fritzen, {Andreas M{\ae}chel} and Annemarie Lundsgaard and Jordy, {Andreas B{\o}rsting} and Poulsen, {Sanne Kellebjerg} and Steen Stender and Henriette Pilegaard and Arne Astrup and Larsen, {Thomas Meinert} and J{\o}rgen Wojtaszewski and Erik Richter and Bente Kiens",

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T1 - New Nordic Diet induced weight loss is accompanied by changes in metabolism and AMPK signalling in adipose tissue

AU - Fritzen, Andreas Mæchel

AU - Lundsgaard, Annemarie

AU - Jordy, Andreas Børsting

AU - Poulsen, Sanne Kellebjerg

AU - Stender, Steen

AU - Pilegaard, Henriette

AU - Astrup, Arne

AU - Larsen, Thomas Meinert

AU - Wojtaszewski, Jørgen

AU - Richter, Erik

AU - Kiens, Bente

N1 - CURIS 2015 NEXS 240

PY - 2015/9/1

Y1 - 2015/9/1

N2 - Context: The molecular mechanisms behind diet-induced metabolic improvements remain to be studied. Objective: This study sought to investigate whether expression of proteins in skeletal muscle or adipose tissue could explain improvements in glucose and lipid homeostasis after weight loss. Design: Volunteers consumed a New Nordic Diet (NND) or an Average Danish Diet for 26 weeks in a controlled, free-living setting. Subjects: Sixty four moderately obese women and men (44 ± 2 y; body mass index, 31 ± 1 kg/m2). Intervention: Fasting blood samples and biopsies from the vastus lateralis muscle and subcutaneous abdominal adipose tissue (SCAT) were obtained at week 0 and 26. Outcome: Gene and protein expressions were analyzed by real-time PCR and Western blotting. Results: Improved homeostasis homeostatic model of assessment-insulin resistance index and lowered plasma triacylglycerol concentration afterNNDcoincided with molecular adaptations in SCAT but not in skeletal muscle. NND induced greater reduction in fat mass than ADD (-6 ± 1 kg and -2 ± 1 kg; P < .01). In SCAT this was associated with increased AMPK and acetyl-CoA carboxylase phosphorylation (P < .05). Concomitantly, NND induced up-regulation of Akt2 and Akt substrate of 160 kDa (P < .05) as well as fatty acid transport protein 4 and membrane associated fatty acid binding protein (P < .05). Indices of increased oxidative capacity were observed, as carnitine palmitoyl transferase 1 mRNA (P = .08) as well as citrate synthase (P = .1) and cytochrome c (P = .05) protein tended to increase. Conclusion: NND-induced metabolic improvements were accompanied by increased AMPK signaling in SCAT, suggesting a role ofAMPKin these adaptations. The concomitant up-regulation of key glucose and lipid-handling proteins suggests an improved metabolic capacity in adipose tissue after weight loss.

AB - Context: The molecular mechanisms behind diet-induced metabolic improvements remain to be studied. Objective: This study sought to investigate whether expression of proteins in skeletal muscle or adipose tissue could explain improvements in glucose and lipid homeostasis after weight loss. Design: Volunteers consumed a New Nordic Diet (NND) or an Average Danish Diet for 26 weeks in a controlled, free-living setting. Subjects: Sixty four moderately obese women and men (44 ± 2 y; body mass index, 31 ± 1 kg/m2). Intervention: Fasting blood samples and biopsies from the vastus lateralis muscle and subcutaneous abdominal adipose tissue (SCAT) were obtained at week 0 and 26. Outcome: Gene and protein expressions were analyzed by real-time PCR and Western blotting. Results: Improved homeostasis homeostatic model of assessment-insulin resistance index and lowered plasma triacylglycerol concentration afterNNDcoincided with molecular adaptations in SCAT but not in skeletal muscle. NND induced greater reduction in fat mass than ADD (-6 ± 1 kg and -2 ± 1 kg; P < .01). In SCAT this was associated with increased AMPK and acetyl-CoA carboxylase phosphorylation (P < .05). Concomitantly, NND induced up-regulation of Akt2 and Akt substrate of 160 kDa (P < .05) as well as fatty acid transport protein 4 and membrane associated fatty acid binding protein (P < .05). Indices of increased oxidative capacity were observed, as carnitine palmitoyl transferase 1 mRNA (P = .08) as well as citrate synthase (P = .1) and cytochrome c (P = .05) protein tended to increase. Conclusion: NND-induced metabolic improvements were accompanied by increased AMPK signaling in SCAT, suggesting a role ofAMPKin these adaptations. The concomitant up-regulation of key glucose and lipid-handling proteins suggests an improved metabolic capacity in adipose tissue after weight loss.

U2 - 10.1210/jc.2015-2079

DO - 10.1210/jc.2015-2079

M3 - Journal article

C2 - 26126206

SN - 0021-972X

VL - 100

SP - 3509

EP - 3519

JO - Journal of Clinical Endocrinology and Metabolism

JF - Journal of Clinical Endocrinology and Metabolism

IS - 9

ER -

New Nordic Diet induced weight loss is accompanied by changes in metabolism and AMPK signalling in adipose tissue

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