New Nordic Diet induced weight loss is accompanied by changes in metabolism and AMPK signalling in adipose tissue

TY - JOUR

T1 - New Nordic Diet induced weight loss is accompanied by changes in metabolism and AMPK signalling in adipose tissue

AU - Fritzen, Andreas Mæchel

AU - Lundsgaard, Annemarie

AU - Jordy, Andreas Børsting

AU - Poulsen, Sanne Kellebjerg

AU - Stender, Steen

AU - Pilegaard, Henriette

AU - Astrup, Arne

AU - Larsen, Thomas Meinert

AU - Wojtaszewski, Jørgen

AU - Richter, Erik

AU - Kiens, Bente

N1 - CURIS 2015 NEXS 240

PY - 2015/9/1

Y1 - 2015/9/1

N2 - Context: The molecular mechanisms behind diet-induced metabolic improvements remain to be studied. Objective: This study sought to investigate whether expression of proteins in skeletal muscle or adipose tissue could explain improvements in glucose and lipid homeostasis after weight loss. Design: Volunteers consumed a New Nordic Diet (NND) or an Average Danish Diet for 26 weeks in a controlled, free-living setting. Subjects: Sixty four moderately obese women and men (44 ± 2 y; body mass index, 31 ± 1 kg/m2). Intervention: Fasting blood samples and biopsies from the vastus lateralis muscle and subcutaneous abdominal adipose tissue (SCAT) were obtained at week 0 and 26. Outcome: Gene and protein expressions were analyzed by real-time PCR and Western blotting. Results: Improved homeostasis homeostatic model of assessment-insulin resistance index and lowered plasma triacylglycerol concentration afterNNDcoincided with molecular adaptations in SCAT but not in skeletal muscle. NND induced greater reduction in fat mass than ADD (-6 ± 1 kg and -2 ± 1 kg; P < .01). In SCAT this was associated with increased AMPK and acetyl-CoA carboxylase phosphorylation (P < .05). Concomitantly, NND induced up-regulation of Akt2 and Akt substrate of 160 kDa (P < .05) as well as fatty acid transport protein 4 and membrane associated fatty acid binding protein (P < .05). Indices of increased oxidative capacity were observed, as carnitine palmitoyl transferase 1 mRNA (P = .08) as well as citrate synthase (P = .1) and cytochrome c (P = .05) protein tended to increase. Conclusion: NND-induced metabolic improvements were accompanied by increased AMPK signaling in SCAT, suggesting a role ofAMPKin these adaptations. The concomitant up-regulation of key glucose and lipid-handling proteins suggests an improved metabolic capacity in adipose tissue after weight loss.

AB - Context: The molecular mechanisms behind diet-induced metabolic improvements remain to be studied. Objective: This study sought to investigate whether expression of proteins in skeletal muscle or adipose tissue could explain improvements in glucose and lipid homeostasis after weight loss. Design: Volunteers consumed a New Nordic Diet (NND) or an Average Danish Diet for 26 weeks in a controlled, free-living setting. Subjects: Sixty four moderately obese women and men (44 ± 2 y; body mass index, 31 ± 1 kg/m2). Intervention: Fasting blood samples and biopsies from the vastus lateralis muscle and subcutaneous abdominal adipose tissue (SCAT) were obtained at week 0 and 26. Outcome: Gene and protein expressions were analyzed by real-time PCR and Western blotting. Results: Improved homeostasis homeostatic model of assessment-insulin resistance index and lowered plasma triacylglycerol concentration afterNNDcoincided with molecular adaptations in SCAT but not in skeletal muscle. NND induced greater reduction in fat mass than ADD (-6 ± 1 kg and -2 ± 1 kg; P < .01). In SCAT this was associated with increased AMPK and acetyl-CoA carboxylase phosphorylation (P < .05). Concomitantly, NND induced up-regulation of Akt2 and Akt substrate of 160 kDa (P < .05) as well as fatty acid transport protein 4 and membrane associated fatty acid binding protein (P < .05). Indices of increased oxidative capacity were observed, as carnitine palmitoyl transferase 1 mRNA (P = .08) as well as citrate synthase (P = .1) and cytochrome c (P = .05) protein tended to increase. Conclusion: NND-induced metabolic improvements were accompanied by increased AMPK signaling in SCAT, suggesting a role ofAMPKin these adaptations. The concomitant up-regulation of key glucose and lipid-handling proteins suggests an improved metabolic capacity in adipose tissue after weight loss.

U2 - 10.1210/jc.2015-2079

DO - 10.1210/jc.2015-2079

M3 - Journal article

C2 - 26126206

SN - 0021-972X

VL - 100

SP - 3509

EP - 3519

JO - Journal of Clinical Endocrinology and Metabolism

JF - Journal of Clinical Endocrinology and Metabolism

IS - 9

ER -