Mutations in the gene for EF-G reduce the requirement for 4.5S RNA in the growth of E. coli

S Brown

Mutations in the gene for EF-G reduce the requirement for 4.5S RNA in the growth of E. coli

Computational and RNA Biology

56 Citations (Scopus)

Abstract

A general strategy is described for the isolation of suppressors of essential genes whose functions are unknown. This strategy was used to analyze the role of 4.5S RNA, an essential RNA of E. coli. In this strategy, the structural gene for 4.5S RNA is fused to the Ptac promoter in such a way that the strain becomes dependent upon inducers of lac for growth. Mutants mapping to fus, the structural gene for protein synthesis elongation factor G, appear as spontaneous, inducer-independent revertants. These mutants alter the intracellular distribution of 4.5S RNA such that it sediments at 70S or greater. Furthermore, the increased sedimentation velocity is sensitive to the antibiotic puromycin. These results show that 4.5S RNA physically associates with the ribosome in performing its essential function, and that this association is mediated by elongation factor G.

Original language	English
Journal	Cell
Volume	49
Issue number	6
Pages (from-to)	825-33
Number of pages	8
ISSN	0092-8674
Publication status	Published - 1987

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@article{e69e4ac0d29011dd9473000ea68e967b,

title = "Mutations in the gene for EF-G reduce the requirement for 4.5S RNA in the growth of E. coli",

abstract = "A general strategy is described for the isolation of suppressors of essential genes whose functions are unknown. This strategy was used to analyze the role of 4.5S RNA, an essential RNA of E. coli. In this strategy, the structural gene for 4.5S RNA is fused to the Ptac promoter in such a way that the strain becomes dependent upon inducers of lac for growth. Mutants mapping to fus, the structural gene for protein synthesis elongation factor G, appear as spontaneous, inducer-independent revertants. These mutants alter the intracellular distribution of 4.5S RNA such that it sediments at 70S or greater. Furthermore, the increased sedimentation velocity is sensitive to the antibiotic puromycin. These results show that 4.5S RNA physically associates with the ribosome in performing its essential function, and that this association is mediated by elongation factor G.",

author = "S Brown",

note = "Keywords: Chromosome Mapping; Escherichia coli; Mutation; Peptide Elongation Factor G; Peptide Elongation Factors; Protein Biosynthesis; RNA, Bacterial; Ribosomes; Suppression, Genetic",

year = "1987",

language = "English",

volume = "49",

pages = "825--33",

journal = "Cell",

issn = "0092-8674",

publisher = "Cell Press",

number = "6",

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T1 - Mutations in the gene for EF-G reduce the requirement for 4.5S RNA in the growth of E. coli

AU - Brown, S

N1 - Keywords: Chromosome Mapping; Escherichia coli; Mutation; Peptide Elongation Factor G; Peptide Elongation Factors; Protein Biosynthesis; RNA, Bacterial; Ribosomes; Suppression, Genetic

PY - 1987

Y1 - 1987

N2 - A general strategy is described for the isolation of suppressors of essential genes whose functions are unknown. This strategy was used to analyze the role of 4.5S RNA, an essential RNA of E. coli. In this strategy, the structural gene for 4.5S RNA is fused to the Ptac promoter in such a way that the strain becomes dependent upon inducers of lac for growth. Mutants mapping to fus, the structural gene for protein synthesis elongation factor G, appear as spontaneous, inducer-independent revertants. These mutants alter the intracellular distribution of 4.5S RNA such that it sediments at 70S or greater. Furthermore, the increased sedimentation velocity is sensitive to the antibiotic puromycin. These results show that 4.5S RNA physically associates with the ribosome in performing its essential function, and that this association is mediated by elongation factor G.

AB - A general strategy is described for the isolation of suppressors of essential genes whose functions are unknown. This strategy was used to analyze the role of 4.5S RNA, an essential RNA of E. coli. In this strategy, the structural gene for 4.5S RNA is fused to the Ptac promoter in such a way that the strain becomes dependent upon inducers of lac for growth. Mutants mapping to fus, the structural gene for protein synthesis elongation factor G, appear as spontaneous, inducer-independent revertants. These mutants alter the intracellular distribution of 4.5S RNA such that it sediments at 70S or greater. Furthermore, the increased sedimentation velocity is sensitive to the antibiotic puromycin. These results show that 4.5S RNA physically associates with the ribosome in performing its essential function, and that this association is mediated by elongation factor G.

M3 - Journal article

C2 - 2438050

SN - 0092-8674

VL - 49

SP - 825

EP - 833

JO - Cell

JF - Cell

IS - 6

ER -

Mutations in the gene for EF-G reduce the requirement for 4.5S RNA in the growth of E. coli

Abstract

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