Mutations in the gene for EF-G reduce the requirement for 4.5S RNA in the growth of E. coli

S Brown

Mutations in the gene for EF-G reduce the requirement for 4.5S RNA in the growth of E. coli

S Brown

Bioinformatik og RNA Biologi

56 Citationer (Scopus)

Abstract

Udgivelsesdato: 1987-Jun-19

Originalsprog	Engelsk
Tidsskrift	Cell
Vol/bind	49
Udgave nummer	6
Sider (fra-til)	825-33
Antal sider	8
ISSN	0092-8674
Status	Udgivet - 1987

Citationsformater

@article{e69e4ac0d29011dd9473000ea68e967b,

title = "Mutations in the gene for EF-G reduce the requirement for 4.5S RNA in the growth of E. coli",

abstract = "A general strategy is described for the isolation of suppressors of essential genes whose functions are unknown. This strategy was used to analyze the role of 4.5S RNA, an essential RNA of E. coli. In this strategy, the structural gene for 4.5S RNA is fused to the Ptac promoter in such a way that the strain becomes dependent upon inducers of lac for growth. Mutants mapping to fus, the structural gene for protein synthesis elongation factor G, appear as spontaneous, inducer-independent revertants. These mutants alter the intracellular distribution of 4.5S RNA such that it sediments at 70S or greater. Furthermore, the increased sedimentation velocity is sensitive to the antibiotic puromycin. These results show that 4.5S RNA physically associates with the ribosome in performing its essential function, and that this association is mediated by elongation factor G.",

author = "S Brown",

note = "Keywords: Chromosome Mapping; Escherichia coli; Mutation; Peptide Elongation Factor G; Peptide Elongation Factors; Protein Biosynthesis; RNA, Bacterial; Ribosomes; Suppression, Genetic",

year = "1987",

language = "English",

volume = "49",

pages = "825--33",

journal = "Cell",

issn = "0092-8674",

publisher = "Cell Press",

number = "6",

}

TY - JOUR

T1 - Mutations in the gene for EF-G reduce the requirement for 4.5S RNA in the growth of E. coli

AU - Brown, S

N1 - Keywords: Chromosome Mapping; Escherichia coli; Mutation; Peptide Elongation Factor G; Peptide Elongation Factors; Protein Biosynthesis; RNA, Bacterial; Ribosomes; Suppression, Genetic

PY - 1987

Y1 - 1987

N2 - A general strategy is described for the isolation of suppressors of essential genes whose functions are unknown. This strategy was used to analyze the role of 4.5S RNA, an essential RNA of E. coli. In this strategy, the structural gene for 4.5S RNA is fused to the Ptac promoter in such a way that the strain becomes dependent upon inducers of lac for growth. Mutants mapping to fus, the structural gene for protein synthesis elongation factor G, appear as spontaneous, inducer-independent revertants. These mutants alter the intracellular distribution of 4.5S RNA such that it sediments at 70S or greater. Furthermore, the increased sedimentation velocity is sensitive to the antibiotic puromycin. These results show that 4.5S RNA physically associates with the ribosome in performing its essential function, and that this association is mediated by elongation factor G.

AB - A general strategy is described for the isolation of suppressors of essential genes whose functions are unknown. This strategy was used to analyze the role of 4.5S RNA, an essential RNA of E. coli. In this strategy, the structural gene for 4.5S RNA is fused to the Ptac promoter in such a way that the strain becomes dependent upon inducers of lac for growth. Mutants mapping to fus, the structural gene for protein synthesis elongation factor G, appear as spontaneous, inducer-independent revertants. These mutants alter the intracellular distribution of 4.5S RNA such that it sediments at 70S or greater. Furthermore, the increased sedimentation velocity is sensitive to the antibiotic puromycin. These results show that 4.5S RNA physically associates with the ribosome in performing its essential function, and that this association is mediated by elongation factor G.

M3 - Journal article

C2 - 2438050

SN - 0092-8674

VL - 49

SP - 825

EP - 833

JO - Cell

JF - Cell

IS - 6

ER -

Mutations in the gene for EF-G reduce the requirement for 4.5S RNA in the growth of E. coli

Abstract

Fingeraftryk

Citationsformater