Mutations in 23S rRNA confer resistance against azithromycin in Pseudomonas aeruginosa

Rasmus Lykke Marvig; Mette S R Søndergaard; Søren Damkiær; Niels Høiby; Helle Krogh Johansen; Søren Molin; Lars Jelsbak

doi:10.1128/AAC.00630-12

Mutations in 23S rRNA confer resistance against azithromycin in Pseudomonas aeruginosa

Rasmus Lykke Marvig, Mette S R Søndergaard, Søren Damkiær, Niels Høiby, Helle Krogh Johansen, Søren Molin, Lars Jelsbak

21 Citations (Scopus)

Abstract

The emergence of antibiotic-resistant Pseudomonas aeruginosa is an important concern in the treatment of long-term airway infections in cystic fibrosis patients. In this study, we report the occurrence of azithromycin resistance among clinical P. aeruginosa DK2 isolates. We demonstrate that resistance is associated with specific mutations (A2058G, A2059G, and C2611T in Escherichia coli numbering) in domain V of 23S rRNA and that introduction of A2058G and C2611T into strain PAO1 results in azithromycin resistance.

Original language	English
Journal	Antimicrobial Agents and Chemotherapy
Volume	56
Issue number	8
Pages (from-to)	4519-4521
Number of pages	3
ISSN	0066-4804
DOIs	https://doi.org/10.1128/AAC.00630-12
Publication status	Published - Aug 2012

Keywords

Anti-Bacterial Agents
Azithromycin
Cystic Fibrosis
Drug Resistance, Bacterial
Humans
Microbial Sensitivity Tests
Mutation
Point Mutation
Pseudomonas Infections
Pseudomonas aeruginosa
RNA, Bacterial
RNA, Ribosomal, 23S

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title = "Mutations in 23S rRNA confer resistance against azithromycin in Pseudomonas aeruginosa",

abstract = "The emergence of antibiotic-resistant Pseudomonas aeruginosa is an important concern in the treatment of long-term airway infections in cystic fibrosis patients. In this study, we report the occurrence of azithromycin resistance among clinical P. aeruginosa DK2 isolates. We demonstrate that resistance is associated with specific mutations (A2058G, A2059G, and C2611T in Escherichia coli numbering) in domain V of 23S rRNA and that introduction of A2058G and C2611T into strain PAO1 results in azithromycin resistance.",

keywords = "Anti-Bacterial Agents, Azithromycin, Cystic Fibrosis, Drug Resistance, Bacterial, Humans, Microbial Sensitivity Tests, Mutation, Point Mutation, Pseudomonas Infections, Pseudomonas aeruginosa, RNA, Bacterial, RNA, Ribosomal, 23S",

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doi = "10.1128/AAC.00630-12",

language = "English",

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T1 - Mutations in 23S rRNA confer resistance against azithromycin in Pseudomonas aeruginosa

AU - Marvig, Rasmus Lykke

AU - Søndergaard, Mette S R

AU - Damkiær, Søren

AU - Høiby, Niels

AU - Johansen, Helle Krogh

AU - Molin, Søren

AU - Jelsbak, Lars

PY - 2012/8

Y1 - 2012/8

N2 - The emergence of antibiotic-resistant Pseudomonas aeruginosa is an important concern in the treatment of long-term airway infections in cystic fibrosis patients. In this study, we report the occurrence of azithromycin resistance among clinical P. aeruginosa DK2 isolates. We demonstrate that resistance is associated with specific mutations (A2058G, A2059G, and C2611T in Escherichia coli numbering) in domain V of 23S rRNA and that introduction of A2058G and C2611T into strain PAO1 results in azithromycin resistance.

AB - The emergence of antibiotic-resistant Pseudomonas aeruginosa is an important concern in the treatment of long-term airway infections in cystic fibrosis patients. In this study, we report the occurrence of azithromycin resistance among clinical P. aeruginosa DK2 isolates. We demonstrate that resistance is associated with specific mutations (A2058G, A2059G, and C2611T in Escherichia coli numbering) in domain V of 23S rRNA and that introduction of A2058G and C2611T into strain PAO1 results in azithromycin resistance.

KW - Anti-Bacterial Agents

KW - Azithromycin

KW - Cystic Fibrosis

KW - Drug Resistance, Bacterial

KW - Humans

KW - Microbial Sensitivity Tests

KW - Mutation

KW - Point Mutation

KW - Pseudomonas Infections

KW - Pseudomonas aeruginosa

KW - RNA, Bacterial

KW - RNA, Ribosomal, 23S

U2 - 10.1128/AAC.00630-12

DO - 10.1128/AAC.00630-12

M3 - Journal article

C2 - 22644032

SN - 0066-4804

VL - 56

SP - 4519

EP - 4521

JO - Antimicrobial Agents and Chemotherapy

JF - Antimicrobial Agents and Chemotherapy

IS - 8

ER -

Mutations in 23S rRNA confer resistance against azithromycin in Pseudomonas aeruginosa

Abstract

Keywords

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