Mutations in 23S rRNA confer resistance against azithromycin in Pseudomonas aeruginosa

TY - JOUR

T1 - Mutations in 23S rRNA confer resistance against azithromycin in Pseudomonas aeruginosa

AU - Marvig, Rasmus Lykke

AU - Søndergaard, Mette S R

AU - Damkiær, Søren

AU - Høiby, Niels

AU - Johansen, Helle Krogh

AU - Molin, Søren

AU - Jelsbak, Lars

PY - 2012/8

Y1 - 2012/8

N2 - The emergence of antibiotic-resistant Pseudomonas aeruginosa is an important concern in the treatment of long-term airway infections in cystic fibrosis patients. In this study, we report the occurrence of azithromycin resistance among clinical P. aeruginosa DK2 isolates. We demonstrate that resistance is associated with specific mutations (A2058G, A2059G, and C2611T in Escherichia coli numbering) in domain V of 23S rRNA and that introduction of A2058G and C2611T into strain PAO1 results in azithromycin resistance.

AB - The emergence of antibiotic-resistant Pseudomonas aeruginosa is an important concern in the treatment of long-term airway infections in cystic fibrosis patients. In this study, we report the occurrence of azithromycin resistance among clinical P. aeruginosa DK2 isolates. We demonstrate that resistance is associated with specific mutations (A2058G, A2059G, and C2611T in Escherichia coli numbering) in domain V of 23S rRNA and that introduction of A2058G and C2611T into strain PAO1 results in azithromycin resistance.

KW - Anti-Bacterial Agents

KW - Azithromycin

KW - Cystic Fibrosis

KW - Drug Resistance, Bacterial

KW - Humans

KW - Microbial Sensitivity Tests

KW - Mutation

KW - Point Mutation

KW - Pseudomonas Infections

KW - Pseudomonas aeruginosa

KW - RNA, Bacterial

KW - RNA, Ribosomal, 23S

U2 - 10.1128/AAC.00630-12

DO - 10.1128/AAC.00630-12

M3 - Journal article

C2 - 22644032

SN - 0066-4804

VL - 56

SP - 4519

EP - 4521

JO - Antimicrobial Agents and Chemotherapy

JF - Antimicrobial Agents and Chemotherapy

IS - 8

ER -