Multiplexed Detection of Autoantibodies to Glycopeptides Using Microarray

Johannes W. Pedersen, Alexander Nøstdal, Hans H. Wandall*

*Corresponding author for this work

Abstract

Protein microarray is a highly sensitive tool for antibody detection in serum. Monitoring of patients’ antibody titers to specific antigens is increasingly employed in the diagnosis of several conditions, ranging from infectious diseases, allergies, autoimmune diseases, and cancer. In this protocol, we present a detailed method for enzymatic generation of disease-specific O-glycopeptides and how to monitor the antibody response to these in serum using microarray technology.

Original languageEnglish
Title of host publicationImmunoproteomics : Methods and Protocols
Number of pages13
PublisherHumana Press
Publication date2019
Pages199-211
ISBN (Print)978-1-4939-9596-7
ISBN (Electronic)978-1-4939-9597-4
DOIs
Publication statusPublished - 2019
SeriesMethods in Molecular Biology
Volume2024
ISSN1064-3745

Keywords

  • Autoantibodies
  • GalNAc
  • Glycopeptide microarray
  • Glycosylation
  • Posttranslational modification
  • Serum biomarkers
  • Sialylation

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