Multiplexed Detection of Autoantibodies to Glycopeptides Using Microarray

Johannes W. Pedersen; Alexander Nøstdal; Hans H. Wandall

doi:10.1007/978-1-4939-9597-4_12

Multiplexed Detection of Autoantibodies to Glycopeptides Using Microarray

Johannes W. Pedersen, Alexander Nøstdal, Hans H. Wandall^*

^*Corresponding author af dette arbejde

Glycomics Program

Abstract

Protein microarray is a highly sensitive tool for antibody detection in serum. Monitoring of patients’ antibody titers to specific antigens is increasingly employed in the diagnosis of several conditions, ranging from infectious diseases, allergies, autoimmune diseases, and cancer. In this protocol, we present a detailed method for enzymatic generation of disease-specific O-glycopeptides and how to monitor the antibody response to these in serum using microarray technology.

Originalsprog	Engelsk
Titel	Immunoproteomics : Methods and Protocols
Antal sider	13
Forlag	Humana Press
Publikationsdato	2019
Sider	199-211
ISBN (Trykt)	978-1-4939-9596-7
ISBN (Elektronisk)	978-1-4939-9597-4
DOI	https://doi.org/10.1007/978-1-4939-9597-4_12
Status	Udgivet - 2019

Navn	Methods in Molecular Biology
Vol/bind	2024
ISSN	1064-3745

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10.1007/978-1-4939-9597-4_12

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keywords = "Autoantibodies, GalNAc, Glycopeptide microarray, Glycosylation, Posttranslational modification, Serum biomarkers, Sialylation",

author = "Pedersen, {Johannes W.} and Alexander N{\o}stdal and Wandall, {Hans H.}",

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AU - Nøstdal, Alexander

AU - Wandall, Hans H.

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N2 - Protein microarray is a highly sensitive tool for antibody detection in serum. Monitoring of patients’ antibody titers to specific antigens is increasingly employed in the diagnosis of several conditions, ranging from infectious diseases, allergies, autoimmune diseases, and cancer. In this protocol, we present a detailed method for enzymatic generation of disease-specific O-glycopeptides and how to monitor the antibody response to these in serum using microarray technology.

AB - Protein microarray is a highly sensitive tool for antibody detection in serum. Monitoring of patients’ antibody titers to specific antigens is increasingly employed in the diagnosis of several conditions, ranging from infectious diseases, allergies, autoimmune diseases, and cancer. In this protocol, we present a detailed method for enzymatic generation of disease-specific O-glycopeptides and how to monitor the antibody response to these in serum using microarray technology.

KW - Autoantibodies

KW - GalNAc

KW - Glycopeptide microarray

KW - Glycosylation

KW - Posttranslational modification

KW - Serum biomarkers

KW - Sialylation

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Multiplexed Detection of Autoantibodies to Glycopeptides Using Microarray

Abstract

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