Multiple roles of Pseudomonas aeruginosa TBCF10839 PilY1 in motility, transport and infection

Yu-Sing Tammy Bohn; Gudrun Brandes; Elza Rakhimova; Sonja Horatzek; Prabhakar Salunkhe; Antje Munder; Andrea van Barneveld; Doris Jordan; Florian Bredenbruch; Susanne Häussler; Kathrin Riedel; Leo Eberl; Peter Østrup Jensen; Thomas Bjarnsholt; Claus Moser; Niels Høiby; Burkhard Tümmler; Lutz Wiehlmann

doi:10.1111/j.1365-2958.2008.06559.x

Multiple roles of Pseudomonas aeruginosa TBCF10839 PilY1 in motility, transport and infection

Yu-Sing Tammy Bohn, Gudrun Brandes, Elza Rakhimova, Sonja Horatzek, Prabhakar Salunkhe, Antje Munder, Andrea van Barneveld, Doris Jordan, Florian Bredenbruch, Susanne Häussler, Kathrin Riedel, Leo Eberl, Peter Østrup Jensen, Thomas Bjarnsholt, Claus Moser, Niels Høiby, Burkhard Tümmler, Lutz Wiehlmann

39 Citations (Scopus)

Abstract

Polymorphonuclear neutrophils are the most important mammalian host defence cells against infections with Pseudomonas aeruginosa. Screening of a signature tagged mutagenesis library of the non-piliated P. aeruginosa strain TBCF10839 uncovered that transposon inactivation of its pilY1 gene rendered the bacterium more resistant against killing by neutrophils than the wild type and any other of the more than 3000 tested mutants. Inactivation of pilY1 led to the loss of twitching motility in twitching-proficient wild-type PA14 and PAO1 strains, predisposed to autolysis and impaired the secretion of quinolones and pyocyanin, but on the other hand promoted growth in stationary phase and bacterial survival in murine airway infection models. The PilY1 population consisted of a major full-length and a minor shorter PilY1* isoform. PilY1* was detectable in small extracellular quinolone-positive aggregates, but not in the pilus. P. aeruginosa PilY1 is not an adhesin on the pilus tip, but assists in pilus biogenesis, twitching motility, secretion of secondary metabolites and in the control of cell density in the bacterial population.

Original language	English
Journal	Molecular Microbiology
Volume	71
Issue number	3
Pages (from-to)	730-47
Number of pages	17
ISSN	0950-382X
DOIs	https://doi.org/10.1111/j.1365-2958.2008.06559.x
Publication status	Published - 2008

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Bohn, Y-S. T., Brandes, G., Rakhimova, E., Horatzek, S., Salunkhe, P., Munder, A., van Barneveld, A., Jordan, D., Bredenbruch, F., Häussler, S., Riedel, K., Eberl, L., Jensen, P. Ø., Bjarnsholt, T., Moser, C., Høiby, N., Tümmler, B., & Wiehlmann, L. (2008). Multiple roles of Pseudomonas aeruginosa TBCF10839 PilY1 in motility, transport and infection. Molecular Microbiology, 71(3), 730-47. https://doi.org/10.1111/j.1365-2958.2008.06559.x

Bohn, Y-ST, Brandes, G, Rakhimova, E, Horatzek, S, Salunkhe, P, Munder, A, van Barneveld, A, Jordan, D, Bredenbruch, F, Häussler, S, Riedel, K, Eberl, L, Jensen, PØ , Bjarnsholt, T, Moser, C, Høiby, N, Tümmler, B & Wiehlmann, L 2008, 'Multiple roles of Pseudomonas aeruginosa TBCF10839 PilY1 in motility, transport and infection', Molecular Microbiology, vol. 71, no. 3, pp. 730-47. https://doi.org/10.1111/j.1365-2958.2008.06559.x

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title = "Multiple roles of Pseudomonas aeruginosa TBCF10839 PilY1 in motility, transport and infection",

abstract = "Polymorphonuclear neutrophils are the most important mammalian host defence cells against infections with Pseudomonas aeruginosa. Screening of a signature tagged mutagenesis library of the non-piliated P. aeruginosa strain TBCF10839 uncovered that transposon inactivation of its pilY1 gene rendered the bacterium more resistant against killing by neutrophils than the wild type and any other of the more than 3000 tested mutants. Inactivation of pilY1 led to the loss of twitching motility in twitching-proficient wild-type PA14 and PAO1 strains, predisposed to autolysis and impaired the secretion of quinolones and pyocyanin, but on the other hand promoted growth in stationary phase and bacterial survival in murine airway infection models. The PilY1 population consisted of a major full-length and a minor shorter PilY1* isoform. PilY1* was detectable in small extracellular quinolone-positive aggregates, but not in the pilus. P. aeruginosa PilY1 is not an adhesin on the pilus tip, but assists in pilus biogenesis, twitching motility, secretion of secondary metabolites and in the control of cell density in the bacterial population.",

author = "Bohn, {Yu-Sing Tammy} and Gudrun Brandes and Elza Rakhimova and Sonja Horatzek and Prabhakar Salunkhe and Antje Munder and {van Barneveld}, Andrea and Doris Jordan and Florian Bredenbruch and Susanne H{\"a}ussler and Kathrin Riedel and Leo Eberl and Jensen, {Peter {\O}strup} and Thomas Bjarnsholt and Claus Moser and Niels H{\o}iby and Burkhard T{\"u}mmler and Lutz Wiehlmann",

note = "Keywords: Animals; Biological Transport; DNA Transposable Elements; DNA, Bacterial; Female; Fimbriae Proteins; Fimbriae, Bacterial; Genes, Bacterial; Mice; Mice, Inbred C3H; Mutagenesis; Neutrophils; Phenazines; Protein Isoforms; Pseudomonas Infections; Pseudomonas aeruginosa; Quinolines; Respiratory Tract Infections",

year = "2008",

doi = "10.1111/j.1365-2958.2008.06559.x",

language = "English",

volume = "71",

pages = "730--47",

journal = "Molecular Microbiology",

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T1 - Multiple roles of Pseudomonas aeruginosa TBCF10839 PilY1 in motility, transport and infection

AU - Bohn, Yu-Sing Tammy

AU - Brandes, Gudrun

AU - Rakhimova, Elza

AU - Horatzek, Sonja

AU - Salunkhe, Prabhakar

AU - Munder, Antje

AU - van Barneveld, Andrea

AU - Jordan, Doris

AU - Bredenbruch, Florian

AU - Häussler, Susanne

AU - Riedel, Kathrin

AU - Eberl, Leo

AU - Jensen, Peter Østrup

AU - Bjarnsholt, Thomas

AU - Moser, Claus

AU - Høiby, Niels

AU - Tümmler, Burkhard

AU - Wiehlmann, Lutz

N1 - Keywords: Animals; Biological Transport; DNA Transposable Elements; DNA, Bacterial; Female; Fimbriae Proteins; Fimbriae, Bacterial; Genes, Bacterial; Mice; Mice, Inbred C3H; Mutagenesis; Neutrophils; Phenazines; Protein Isoforms; Pseudomonas Infections; Pseudomonas aeruginosa; Quinolines; Respiratory Tract Infections

PY - 2008

Y1 - 2008

N2 - Polymorphonuclear neutrophils are the most important mammalian host defence cells against infections with Pseudomonas aeruginosa. Screening of a signature tagged mutagenesis library of the non-piliated P. aeruginosa strain TBCF10839 uncovered that transposon inactivation of its pilY1 gene rendered the bacterium more resistant against killing by neutrophils than the wild type and any other of the more than 3000 tested mutants. Inactivation of pilY1 led to the loss of twitching motility in twitching-proficient wild-type PA14 and PAO1 strains, predisposed to autolysis and impaired the secretion of quinolones and pyocyanin, but on the other hand promoted growth in stationary phase and bacterial survival in murine airway infection models. The PilY1 population consisted of a major full-length and a minor shorter PilY1* isoform. PilY1* was detectable in small extracellular quinolone-positive aggregates, but not in the pilus. P. aeruginosa PilY1 is not an adhesin on the pilus tip, but assists in pilus biogenesis, twitching motility, secretion of secondary metabolites and in the control of cell density in the bacterial population.

AB - Polymorphonuclear neutrophils are the most important mammalian host defence cells against infections with Pseudomonas aeruginosa. Screening of a signature tagged mutagenesis library of the non-piliated P. aeruginosa strain TBCF10839 uncovered that transposon inactivation of its pilY1 gene rendered the bacterium more resistant against killing by neutrophils than the wild type and any other of the more than 3000 tested mutants. Inactivation of pilY1 led to the loss of twitching motility in twitching-proficient wild-type PA14 and PAO1 strains, predisposed to autolysis and impaired the secretion of quinolones and pyocyanin, but on the other hand promoted growth in stationary phase and bacterial survival in murine airway infection models. The PilY1 population consisted of a major full-length and a minor shorter PilY1* isoform. PilY1* was detectable in small extracellular quinolone-positive aggregates, but not in the pilus. P. aeruginosa PilY1 is not an adhesin on the pilus tip, but assists in pilus biogenesis, twitching motility, secretion of secondary metabolites and in the control of cell density in the bacterial population.

U2 - 10.1111/j.1365-2958.2008.06559.x

DO - 10.1111/j.1365-2958.2008.06559.x

M3 - Journal article

C2 - 19054330

SN - 0950-382X

VL - 71

SP - 730

EP - 747

JO - Molecular Microbiology

JF - Molecular Microbiology

IS - 3

ER -

Multiple roles of Pseudomonas aeruginosa TBCF10839 PilY1 in motility, transport and infection

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