TY - JOUR
T1 - Multiple roles of Pseudomonas aeruginosa TBCF10839 PilY1 in motility, transport and infection
AU - Bohn, Yu-Sing Tammy
AU - Brandes, Gudrun
AU - Rakhimova, Elza
AU - Horatzek, Sonja
AU - Salunkhe, Prabhakar
AU - Munder, Antje
AU - van Barneveld, Andrea
AU - Jordan, Doris
AU - Bredenbruch, Florian
AU - Häussler, Susanne
AU - Riedel, Kathrin
AU - Eberl, Leo
AU - Jensen, Peter Østrup
AU - Bjarnsholt, Thomas
AU - Moser, Claus
AU - Høiby, Niels
AU - Tümmler, Burkhard
AU - Wiehlmann, Lutz
N1 - Keywords: Animals; Biological Transport; DNA Transposable Elements; DNA, Bacterial; Female; Fimbriae Proteins; Fimbriae, Bacterial; Genes, Bacterial; Mice; Mice, Inbred C3H; Mutagenesis; Neutrophils; Phenazines; Protein Isoforms; Pseudomonas Infections; Pseudomonas aeruginosa; Quinolines; Respiratory Tract Infections
PY - 2008
Y1 - 2008
N2 - Polymorphonuclear neutrophils are the most important mammalian host defence cells against infections with Pseudomonas aeruginosa. Screening of a signature tagged mutagenesis library of the non-piliated P. aeruginosa strain TBCF10839 uncovered that transposon inactivation of its pilY1 gene rendered the bacterium more resistant against killing by neutrophils than the wild type and any other of the more than 3000 tested mutants. Inactivation of pilY1 led to the loss of twitching motility in twitching-proficient wild-type PA14 and PAO1 strains, predisposed to autolysis and impaired the secretion of quinolones and pyocyanin, but on the other hand promoted growth in stationary phase and bacterial survival in murine airway infection models. The PilY1 population consisted of a major full-length and a minor shorter PilY1* isoform. PilY1* was detectable in small extracellular quinolone-positive aggregates, but not in the pilus. P. aeruginosa PilY1 is not an adhesin on the pilus tip, but assists in pilus biogenesis, twitching motility, secretion of secondary metabolites and in the control of cell density in the bacterial population.
AB - Polymorphonuclear neutrophils are the most important mammalian host defence cells against infections with Pseudomonas aeruginosa. Screening of a signature tagged mutagenesis library of the non-piliated P. aeruginosa strain TBCF10839 uncovered that transposon inactivation of its pilY1 gene rendered the bacterium more resistant against killing by neutrophils than the wild type and any other of the more than 3000 tested mutants. Inactivation of pilY1 led to the loss of twitching motility in twitching-proficient wild-type PA14 and PAO1 strains, predisposed to autolysis and impaired the secretion of quinolones and pyocyanin, but on the other hand promoted growth in stationary phase and bacterial survival in murine airway infection models. The PilY1 population consisted of a major full-length and a minor shorter PilY1* isoform. PilY1* was detectable in small extracellular quinolone-positive aggregates, but not in the pilus. P. aeruginosa PilY1 is not an adhesin on the pilus tip, but assists in pilus biogenesis, twitching motility, secretion of secondary metabolites and in the control of cell density in the bacterial population.
U2 - 10.1111/j.1365-2958.2008.06559.x
DO - 10.1111/j.1365-2958.2008.06559.x
M3 - Journal article
C2 - 19054330
SN - 0950-382X
VL - 71
SP - 730
EP - 747
JO - Molecular Microbiology
JF - Molecular Microbiology
IS - 3
ER -