mRNA profiling for the identification of blood--Results of a collaborative EDNAP exercise

C. Haas; E. Hanson; W. Bär; R. Banemann; A.M. Bento; A. Berti; E. Borges; C. Bouakaze; A. Carracedo; M. Carvalho; A. Choma; M. Dötsch; M. Durianciková; P. Hoff-Olsen; C. Hohoff; P. Johansen; P.A. Lindenbergh; B. Loddenkötter; B. Ludes; O. Maroñas; N. Morling; H. Niederstätter; W. Parson; G. Patel; C. Popielarz; E. Salata; P.M. Schneider; T. Sijen; B. Sviezená; L. Zatkalíková; J. Ballantyne

doi:10.1016/j.fsigen.2010.01.003

mRNA profiling for the identification of blood--Results of a collaborative EDNAP exercise

C. Haas, E. Hanson, W. Bär, R. Banemann, A.M. Bento, A. Berti, E. Borges, C. Bouakaze, A. Carracedo, M. Carvalho, A. Choma, M. Dötsch, M. Durianciková, P. Hoff-Olsen, C. Hohoff, P. Johansen, P.A. Lindenbergh, B. Loddenkötter, B. Ludes, O. MaroñasN. Morling, H. Niederstätter, W. Parson, G. Patel, C. Popielarz, E. Salata, P.M. Schneider, T. Sijen, B. Sviezená, L. Zatkalíková, J. Ballantyne

84 Citations (Scopus)

Abstract

A collaborative exercise on mRNA profiling for the identification of blood was organized by the European DNA Profiling Group (EDNAP). Seven blood samples and one blood dilution series were analyzed by the participating laboratories for the reportedly blood-specific markers HBB, SPTB and PBGD, using different kits, chemistries and instrumentation. The results demonstrate that HBB is expressed abundantly in blood, SPTB moderately and PBGD significantly less. All but one of the 16 participating laboratories were able to successfully isolate and detect RNA from the dried bloodstains even though a majority of the laboratories had no prior experience with RNA. Despite some expected variation in sensitivity between laboratories, the method proved to be reproducible and sensitive using different analysis strategies. The results of this collaborative exercise support the potential use of mRNA profiling as an alternative to conventional serological tests.

Translated title of the contribution	mRNA profiling for the identification of blood--Results of a collaborative EDNAP exercise
Original language	English
Journal	Forensic Science International: Genetics
Volume	5
Issue number	1
Pages (from-to)	21 - 26
Number of pages	6
ISSN	1872-4973
DOIs	https://doi.org/10.1016/j.fsigen.2010.01.003
Publication status	Published - Jan 2011

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10.1016/j.fsigen.2010.01.003

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Haas, C., Hanson, E., Bär, W., Banemann, R., Bento, A. M., Berti, A., Borges, E., Bouakaze, C., Carracedo, A., Carvalho, M., Choma, A., Dötsch, M., Durianciková, M., Hoff-Olsen, P., Hohoff, C., Johansen, P., Lindenbergh, P. A., Loddenkötter, B., Ludes, B., ... Ballantyne, J. (2011). mRNA profiling for the identification of blood--Results of a collaborative EDNAP exercise. Forensic Science International: Genetics, 5(1), 21 - 26. https://doi.org/10.1016/j.fsigen.2010.01.003

Haas, C, Hanson, E, Bär, W, Banemann, R, Bento, AM, Berti, A, Borges, E, Bouakaze, C, Carracedo, A, Carvalho, M, Choma, A, Dötsch, M, Durianciková, M, Hoff-Olsen, P, Hohoff, C, Johansen, P, Lindenbergh, PA, Loddenkötter, B, Ludes, B, Maroñas, O, Morling, N, Niederstätter, H, Parson, W, Patel, G, Popielarz, C, Salata, E, Schneider, PM, Sijen, T, Sviezená, B, Zatkalíková, L & Ballantyne, J 2011, 'mRNA profiling for the identification of blood--Results of a collaborative EDNAP exercise', Forensic Science International: Genetics, vol. 5, no. 1, pp. 21 - 26. https://doi.org/10.1016/j.fsigen.2010.01.003

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abstract = "A collaborative exercise on mRNA profiling for the identification of blood was organized by the European DNA Profiling Group (EDNAP). Seven blood samples and one blood dilution series were analyzed by the participating laboratories for the reportedly blood-specific markers HBB, SPTB and PBGD, using different kits, chemistries and instrumentation. The results demonstrate that HBB is expressed abundantly in blood, SPTB moderately and PBGD significantly less. All but one of the 16 participating laboratories were able to successfully isolate and detect RNA from the dried bloodstains even though a majority of the laboratories had no prior experience with RNA. Despite some expected variation in sensitivity between laboratories, the method proved to be reproducible and sensitive using different analysis strategies. The results of this collaborative exercise support the potential use of mRNA profiling as an alternative to conventional serological tests.",

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author = "C. Haas and E. Hanson and W. B{\"a}r and R. Banemann and A.M. Bento and A. Berti and E. Borges and C. Bouakaze and A. Carracedo and M. Carvalho and A. Choma and M. D{\"o}tsch and M. Duriancikov{\'a} and P. Hoff-Olsen and C. Hohoff and P. Johansen and P.A. Lindenbergh and B. Loddenk{\"o}tter and B. Ludes and O. Maro{\~n}as and N. Morling and H. Niederst{\"a}tter and W. Parson and G. Patel and C. Popielarz and E. Salata and P.M. Schneider and T. Sijen and B. Sviezen{\'a} and L. Zatkal{\'i}kov{\'a} and J. Ballantyne",

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AU - Haas, C.

AU - Hanson, E.

AU - Bär, W.

AU - Banemann, R.

AU - Bento, A.M.

AU - Berti, A.

AU - Borges, E.

AU - Bouakaze, C.

AU - Carracedo, A.

AU - Carvalho, M.

AU - Choma, A.

AU - Dötsch, M.

AU - Durianciková, M.

AU - Hoff-Olsen, P.

AU - Hohoff, C.

AU - Johansen, P.

AU - Lindenbergh, P.A.

AU - Loddenkötter, B.

AU - Ludes, B.

AU - Maroñas, O.

AU - Morling, N.

AU - Niederstätter, H.

AU - Parson, W.

AU - Patel, G.

AU - Popielarz, C.

AU - Salata, E.

AU - Schneider, P.M.

AU - Sijen, T.

AU - Sviezená, B.

AU - Zatkalíková, L.

AU - Ballantyne, J.

PY - 2011/1

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N2 - A collaborative exercise on mRNA profiling for the identification of blood was organized by the European DNA Profiling Group (EDNAP). Seven blood samples and one blood dilution series were analyzed by the participating laboratories for the reportedly blood-specific markers HBB, SPTB and PBGD, using different kits, chemistries and instrumentation. The results demonstrate that HBB is expressed abundantly in blood, SPTB moderately and PBGD significantly less. All but one of the 16 participating laboratories were able to successfully isolate and detect RNA from the dried bloodstains even though a majority of the laboratories had no prior experience with RNA. Despite some expected variation in sensitivity between laboratories, the method proved to be reproducible and sensitive using different analysis strategies. The results of this collaborative exercise support the potential use of mRNA profiling as an alternative to conventional serological tests.

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