Mouse oocyte meiosis is disturbed by knockdown of Suv4-20h

Kai Xiong; Wu Wei; Xuguang Wang; Xueshan Ma; Jie Chen; Honglin Liu

Mouse oocyte meiosis is disturbed by knockdown of Suv4-20h

Kai Xiong, Wu Wei, Xuguang Wang, Xueshan Ma, Jie Chen, Honglin Liu

3 Citations (Scopus)

Abstract

Suv4-20h was initially characterised as a histone methyltransferase (HMTase) that catalyses lysine 20 of histone H4 dimethylation (H4K20me2) and trimethylation (H4K20me3). In the present study, using RNA interference (RNAi), we found that Suv4-20h activity is required for the fidelity of chromosome distribution during meiosis in the mammalian oocyte. Knockdown of Suv4-20h resulted in attenuation of H4K20me3 and the accumulation of H4K20me1. After Suv4-20h knockdown, oocytes exhibited an increasing percentage of aberrant chromosome alignment in MI, together with a decreasing percentage of polar body I extrusion. We conclude that Suv4-20h may be required for normal chromosome behaviour and that it is crucial for proper meiotic progression in mammalian oocytes.

Original language	English
Journal	Reproduction, Fertility and Development
Volume	25
Issue number	3
Pages (from-to)	503–510
ISSN	1031-3613
Publication status	Published - 2013
Externally published	Yes

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title = "Mouse oocyte meiosis is disturbed by knockdown of Suv4-20h",

abstract = "Suv4-20h was initially characterised as a histone methyltransferase (HMTase) that catalyses lysine 20 of histone H4 dimethylation (H4K20me2) and trimethylation (H4K20me3). In the present study, using RNA interference (RNAi), we found that Suv4-20h activity is required for the fidelity of chromosome distribution during meiosis in the mammalian oocyte. Knockdown of Suv4-20h resulted in attenuation of H4K20me3 and the accumulation of H4K20me1. After Suv4-20h knockdown, oocytes exhibited an increasing percentage of aberrant chromosome alignment in MI, together with a decreasing percentage of polar body I extrusion. We conclude that Suv4-20h may be required for normal chromosome behaviour and that it is crucial for proper meiotic progression in mammalian oocytes.",

author = "Kai Xiong and Wu Wei and Xuguang Wang and Xueshan Ma and Jie Chen and Honglin Liu",

year = "2013",

language = "English",

volume = "25",

pages = "503–510",

journal = "Australian journal of scientific research. Ser. B: Biological sciences",

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TY - JOUR

T1 - Mouse oocyte meiosis is disturbed by knockdown of Suv4-20h

AU - Xiong, Kai

AU - Wei, Wu

AU - Wang, Xuguang

AU - Ma, Xueshan

AU - Chen, Jie

AU - Liu, Honglin

PY - 2013

Y1 - 2013

N2 - Suv4-20h was initially characterised as a histone methyltransferase (HMTase) that catalyses lysine 20 of histone H4 dimethylation (H4K20me2) and trimethylation (H4K20me3). In the present study, using RNA interference (RNAi), we found that Suv4-20h activity is required for the fidelity of chromosome distribution during meiosis in the mammalian oocyte. Knockdown of Suv4-20h resulted in attenuation of H4K20me3 and the accumulation of H4K20me1. After Suv4-20h knockdown, oocytes exhibited an increasing percentage of aberrant chromosome alignment in MI, together with a decreasing percentage of polar body I extrusion. We conclude that Suv4-20h may be required for normal chromosome behaviour and that it is crucial for proper meiotic progression in mammalian oocytes.

AB - Suv4-20h was initially characterised as a histone methyltransferase (HMTase) that catalyses lysine 20 of histone H4 dimethylation (H4K20me2) and trimethylation (H4K20me3). In the present study, using RNA interference (RNAi), we found that Suv4-20h activity is required for the fidelity of chromosome distribution during meiosis in the mammalian oocyte. Knockdown of Suv4-20h resulted in attenuation of H4K20me3 and the accumulation of H4K20me1. After Suv4-20h knockdown, oocytes exhibited an increasing percentage of aberrant chromosome alignment in MI, together with a decreasing percentage of polar body I extrusion. We conclude that Suv4-20h may be required for normal chromosome behaviour and that it is crucial for proper meiotic progression in mammalian oocytes.

M3 - Journal article

SN - 1031-3613

VL - 25

SP - 503

EP - 510

JO - Australian journal of scientific research. Ser. B: Biological sciences

JF - Australian journal of scientific research. Ser. B: Biological sciences

IS - 3

ER -

Mouse oocyte meiosis is disturbed by knockdown of Suv4-20h

Abstract

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