Mouse oocyte meiosis is disturbed by knockdown of Suv4-20h

Kai Xiong; Wu Wei; Xuguang Wang; Xueshan Ma; Jie Chen; Honglin Liu

Mouse oocyte meiosis is disturbed by knockdown of Suv4-20h

Kai Xiong, Wu Wei, Xuguang Wang, Xueshan Ma, Jie Chen, Honglin Liu

3 Citationer (Scopus)

Abstract

Suv4-20h was initially characterised as a histone methyltransferase (HMTase) that catalyses lysine 20 of histone H4 dimethylation (H4K20me2) and trimethylation (H4K20me3). In the present study, using RNA interference (RNAi), we found that Suv4-20h activity is required for the fidelity of chromosome distribution during meiosis in the mammalian oocyte. Knockdown of Suv4-20h resulted in attenuation of H4K20me3 and the accumulation of H4K20me1. After Suv4-20h knockdown, oocytes exhibited an increasing percentage of aberrant chromosome alignment in MI, together with a decreasing percentage of polar body I extrusion. We conclude that Suv4-20h may be required for normal chromosome behaviour and that it is crucial for proper meiotic progression in mammalian oocytes.

Originalsprog	Engelsk
Tidsskrift	Reproduction, Fertility and Development
Vol/bind	25
Udgave nummer	3
Sider (fra-til)	503–510
ISSN	1031-3613
Status	Udgivet - 2013
Udgivet eksternt	Ja

Citationsformater

@article{b1f5a4bab48e47ed87a3c74626aa6e0a,

title = "Mouse oocyte meiosis is disturbed by knockdown of Suv4-20h",

abstract = "Suv4-20h was initially characterised as a histone methyltransferase (HMTase) that catalyses lysine 20 of histone H4 dimethylation (H4K20me2) and trimethylation (H4K20me3). In the present study, using RNA interference (RNAi), we found that Suv4-20h activity is required for the fidelity of chromosome distribution during meiosis in the mammalian oocyte. Knockdown of Suv4-20h resulted in attenuation of H4K20me3 and the accumulation of H4K20me1. After Suv4-20h knockdown, oocytes exhibited an increasing percentage of aberrant chromosome alignment in MI, together with a decreasing percentage of polar body I extrusion. We conclude that Suv4-20h may be required for normal chromosome behaviour and that it is crucial for proper meiotic progression in mammalian oocytes.",

author = "Kai Xiong and Wu Wei and Xuguang Wang and Xueshan Ma and Jie Chen and Honglin Liu",

year = "2013",

language = "English",

volume = "25",

pages = "503–510",

journal = "Australian journal of scientific research. Ser. B: Biological sciences",

issn = "1031-3613",

publisher = "C S I R O Publishing",

number = "3",

}

TY - JOUR

T1 - Mouse oocyte meiosis is disturbed by knockdown of Suv4-20h

AU - Xiong, Kai

AU - Wei, Wu

AU - Wang, Xuguang

AU - Ma, Xueshan

AU - Chen, Jie

AU - Liu, Honglin

PY - 2013

Y1 - 2013

N2 - Suv4-20h was initially characterised as a histone methyltransferase (HMTase) that catalyses lysine 20 of histone H4 dimethylation (H4K20me2) and trimethylation (H4K20me3). In the present study, using RNA interference (RNAi), we found that Suv4-20h activity is required for the fidelity of chromosome distribution during meiosis in the mammalian oocyte. Knockdown of Suv4-20h resulted in attenuation of H4K20me3 and the accumulation of H4K20me1. After Suv4-20h knockdown, oocytes exhibited an increasing percentage of aberrant chromosome alignment in MI, together with a decreasing percentage of polar body I extrusion. We conclude that Suv4-20h may be required for normal chromosome behaviour and that it is crucial for proper meiotic progression in mammalian oocytes.

AB - Suv4-20h was initially characterised as a histone methyltransferase (HMTase) that catalyses lysine 20 of histone H4 dimethylation (H4K20me2) and trimethylation (H4K20me3). In the present study, using RNA interference (RNAi), we found that Suv4-20h activity is required for the fidelity of chromosome distribution during meiosis in the mammalian oocyte. Knockdown of Suv4-20h resulted in attenuation of H4K20me3 and the accumulation of H4K20me1. After Suv4-20h knockdown, oocytes exhibited an increasing percentage of aberrant chromosome alignment in MI, together with a decreasing percentage of polar body I extrusion. We conclude that Suv4-20h may be required for normal chromosome behaviour and that it is crucial for proper meiotic progression in mammalian oocytes.

M3 - Journal article

SN - 1031-3613

VL - 25

SP - 503

EP - 510

JO - Australian journal of scientific research. Ser. B: Biological sciences

JF - Australian journal of scientific research. Ser. B: Biological sciences

IS - 3

ER -

Mouse oocyte meiosis is disturbed by knockdown of Suv4-20h

Abstract

Fingeraftryk

Citationsformater