Morphology of Entomophthora muscae protoplasts grown in vitro

J. P. Latgé; J. Eilenberg; A. Beauvais; M. C. Prevost

doi:10.1007/BF01405926

Morphology of Entomophthora muscae protoplasts grown in vitro

J. P. Latgé^*, J. Eilenberg, A. Beauvais, M. C. Prevost

^*Corresponding author for this work

Section for Organismal Biology

15 Citations (Scopus)

Abstract

Entomophthora muscae (C.) Fres. can be grown in vitro as protoplasts. Light and electron microscopical studies of the in vitro developed protoplasts have demonstrated the absence of an organized wall over the protoplasmic Con A-positive membrane at all stages of growth. The cytological organization is typical of the Entomophthorales with condensed chromatin in the interphase nuclei and small eccentric metaphase spindles. Long strands of endoplasmic reticulum, microubules and vesicles surrounding the plasmalemma may be involved in maintaining the precise shape of E. muscae protoplast. Starvation of the fungus induces the formation of hyphal bodies after deposition of Con A- and WGA-positive wall material at the plasmalemma surface.

Original language	English
Journal	Protoplasma
Volume	146
Issue number	2-3
Pages (from-to)	166-173
Number of pages	8
ISSN	0033-183X
DOIs	https://doi.org/10.1007/BF01405926
Publication status	Published - 1 Jun 1988

Keywords

Cytochemistry
Entomophthora muscae
Entomophthorales
in vitro growth
Protoplast
Ultrastructure

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10.1007/BF01405926

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title = "Morphology of Entomophthora muscae protoplasts grown in vitro",

abstract = "Entomophthora muscae (C.) Fres. can be grown in vitro as protoplasts. Light and electron microscopical studies of the in vitro developed protoplasts have demonstrated the absence of an organized wall over the protoplasmic Con A-positive membrane at all stages of growth. The cytological organization is typical of the Entomophthorales with condensed chromatin in the interphase nuclei and small eccentric metaphase spindles. Long strands of endoplasmic reticulum, microubules and vesicles surrounding the plasmalemma may be involved in maintaining the precise shape of E. muscae protoplast. Starvation of the fungus induces the formation of hyphal bodies after deposition of Con A- and WGA-positive wall material at the plasmalemma surface.",

keywords = "Cytochemistry, Entomophthora muscae, Entomophthorales, in vitro growth, Protoplast, Ultrastructure",

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TY - JOUR

T1 - Morphology of Entomophthora muscae protoplasts grown in vitro

AU - Latgé, J. P.

AU - Eilenberg, J.

AU - Beauvais, A.

AU - Prevost, M. C.

PY - 1988/6/1

Y1 - 1988/6/1

N2 - Entomophthora muscae (C.) Fres. can be grown in vitro as protoplasts. Light and electron microscopical studies of the in vitro developed protoplasts have demonstrated the absence of an organized wall over the protoplasmic Con A-positive membrane at all stages of growth. The cytological organization is typical of the Entomophthorales with condensed chromatin in the interphase nuclei and small eccentric metaphase spindles. Long strands of endoplasmic reticulum, microubules and vesicles surrounding the plasmalemma may be involved in maintaining the precise shape of E. muscae protoplast. Starvation of the fungus induces the formation of hyphal bodies after deposition of Con A- and WGA-positive wall material at the plasmalemma surface.

AB - Entomophthora muscae (C.) Fres. can be grown in vitro as protoplasts. Light and electron microscopical studies of the in vitro developed protoplasts have demonstrated the absence of an organized wall over the protoplasmic Con A-positive membrane at all stages of growth. The cytological organization is typical of the Entomophthorales with condensed chromatin in the interphase nuclei and small eccentric metaphase spindles. Long strands of endoplasmic reticulum, microubules and vesicles surrounding the plasmalemma may be involved in maintaining the precise shape of E. muscae protoplast. Starvation of the fungus induces the formation of hyphal bodies after deposition of Con A- and WGA-positive wall material at the plasmalemma surface.

KW - Cytochemistry

KW - Entomophthora muscae

KW - Entomophthorales

KW - in vitro growth

KW - Protoplast

KW - Ultrastructure

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DO - 10.1007/BF01405926

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VL - 146

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EP - 173

JO - Protoplasma

JF - Protoplasma

IS - 2-3

ER -

Morphology of Entomophthora muscae protoplasts grown in vitro

Abstract

Keywords

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