Morphology of Entomophthora muscae protoplasts grown in vitro

J. P. Latgé; J. Eilenberg; A. Beauvais; M. C. Prevost

doi:10.1007/BF01405926

Morphology of Entomophthora muscae protoplasts grown in vitro

J. P. Latgé^*, J. Eilenberg, A. Beauvais, M. C. Prevost

^*Corresponding author af dette arbejde

SOBI

15 Citationer (Scopus)

Abstract

Entomophthora muscae (C.) Fres. can be grown in vitro as protoplasts. Light and electron microscopical studies of the in vitro developed protoplasts have demonstrated the absence of an organized wall over the protoplasmic Con A-positive membrane at all stages of growth. The cytological organization is typical of the Entomophthorales with condensed chromatin in the interphase nuclei and small eccentric metaphase spindles. Long strands of endoplasmic reticulum, microubules and vesicles surrounding the plasmalemma may be involved in maintaining the precise shape of E. muscae protoplast. Starvation of the fungus induces the formation of hyphal bodies after deposition of Con A- and WGA-positive wall material at the plasmalemma surface.

Originalsprog	Engelsk
Tidsskrift	Protoplasma
Vol/bind	146
Udgave nummer	2-3
Sider (fra-til)	166-173
Antal sider	8
ISSN	0033-183X
DOI	https://doi.org/10.1007/BF01405926
Status	Udgivet - 1 jun. 1988

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title = "Morphology of Entomophthora muscae protoplasts grown in vitro",

abstract = "Entomophthora muscae (C.) Fres. can be grown in vitro as protoplasts. Light and electron microscopical studies of the in vitro developed protoplasts have demonstrated the absence of an organized wall over the protoplasmic Con A-positive membrane at all stages of growth. The cytological organization is typical of the Entomophthorales with condensed chromatin in the interphase nuclei and small eccentric metaphase spindles. Long strands of endoplasmic reticulum, microubules and vesicles surrounding the plasmalemma may be involved in maintaining the precise shape of E. muscae protoplast. Starvation of the fungus induces the formation of hyphal bodies after deposition of Con A- and WGA-positive wall material at the plasmalemma surface.",

keywords = "Cytochemistry, Entomophthora muscae, Entomophthorales, in vitro growth, Protoplast, Ultrastructure",

author = "Latg{\'e}, {J. P.} and J. Eilenberg and A. Beauvais and Prevost, {M. C.}",

year = "1988",

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doi = "10.1007/BF01405926",

language = "English",

volume = "146",

pages = "166--173",

journal = "Protoplasma",

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TY - JOUR

T1 - Morphology of Entomophthora muscae protoplasts grown in vitro

AU - Latgé, J. P.

AU - Eilenberg, J.

AU - Beauvais, A.

AU - Prevost, M. C.

PY - 1988/6/1

Y1 - 1988/6/1

N2 - Entomophthora muscae (C.) Fres. can be grown in vitro as protoplasts. Light and electron microscopical studies of the in vitro developed protoplasts have demonstrated the absence of an organized wall over the protoplasmic Con A-positive membrane at all stages of growth. The cytological organization is typical of the Entomophthorales with condensed chromatin in the interphase nuclei and small eccentric metaphase spindles. Long strands of endoplasmic reticulum, microubules and vesicles surrounding the plasmalemma may be involved in maintaining the precise shape of E. muscae protoplast. Starvation of the fungus induces the formation of hyphal bodies after deposition of Con A- and WGA-positive wall material at the plasmalemma surface.

AB - Entomophthora muscae (C.) Fres. can be grown in vitro as protoplasts. Light and electron microscopical studies of the in vitro developed protoplasts have demonstrated the absence of an organized wall over the protoplasmic Con A-positive membrane at all stages of growth. The cytological organization is typical of the Entomophthorales with condensed chromatin in the interphase nuclei and small eccentric metaphase spindles. Long strands of endoplasmic reticulum, microubules and vesicles surrounding the plasmalemma may be involved in maintaining the precise shape of E. muscae protoplast. Starvation of the fungus induces the formation of hyphal bodies after deposition of Con A- and WGA-positive wall material at the plasmalemma surface.

KW - Cytochemistry

KW - Entomophthora muscae

KW - Entomophthorales

KW - in vitro growth

KW - Protoplast

KW - Ultrastructure

UR - http://www.scopus.com/inward/record.url?scp=0008584401&partnerID=8YFLogxK

U2 - 10.1007/BF01405926

DO - 10.1007/BF01405926

M3 - Journal article

AN - SCOPUS:0008584401

SN - 0033-183X

VL - 146

SP - 166

EP - 173

JO - Protoplasma

JF - Protoplasma

IS - 2-3

ER -

Morphology of Entomophthora muscae protoplasts grown in vitro

Abstract

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