Molecular markers for tolerance of European ash (Fraxinus excelsior) to dieback disease identified using Associative Transcriptomics

Andrea L. Harper; Lea Vig McKinney; Lene Rostgaard Nielsen; Lenka Havlickova; Yi Li; Martin Trick; Fiona Fraser; Lihong Wang; Alison Fellgett; Elizabeth S A Sollars; Sophie H. Janacek; J. Allan Downie; Richard J A Buggs; Erik Dahl Kjær; Ian Bancroft

doi:10.1038/srep19335

Molecular markers for tolerance of European ash (Fraxinus excelsior) to dieback disease identified using Associative Transcriptomics

Andrea L. Harper^*, Lea Vig McKinney, Lene Rostgaard Nielsen, Lenka Havlickova, Yi Li, Martin Trick, Fiona Fraser, Lihong Wang, Alison Fellgett, Elizabeth S A Sollars, Sophie H. Janacek, J. Allan Downie, Richard J A Buggs, Erik Dahl Kjær, Ian Bancroft

^*Corresponding author for this work

Forest, Nature and Biomass

54 Citations (Scopus)

Abstract

Tree disease epidemics are a global problem, impacting food security, biodiversity and national economies. The potential for conservation and breeding in trees is hampered by complex genomes and long lifecycles, with most species lacking genomic resources. The European Ash tree Fraxinus excelsior is being devastated by the fungal pathogen Hymenoscyphus fraxineus, which causes ash dieback disease. Taking this system as an example and utilizing Associative Transcriptomics for the first time in a plant pathology study, we discovered gene sequence and gene expression variants across a genetic diversity panel scored for disease symptoms and identified markers strongly associated with canopy damage in infected trees. Using these markers we predicted phenotypes in a test panel of unrelated trees, successfully identifying individuals with a low level of susceptibility to the disease. Co-expression analysis suggested that pre-priming of defence responses may underlie reduced susceptibility to ash dieback.

Original language	English
Article number	19335
Journal	Scientific Reports
Volume	6
ISSN	2045-2322
DOIs	https://doi.org/10.1038/srep19335
Publication status	Published - 13 Jan 2016

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Harper, A. L., McKinney, L. V., Nielsen, L. R., Havlickova, L., Li, Y., Trick, M., Fraser, F., Wang, L., Fellgett, A., Sollars, E. S. A., Janacek, S. H., Downie, J. A., Buggs, R. J. A., Kjær, E. D., & Bancroft, I. (2016). Molecular markers for tolerance of European ash (Fraxinus excelsior) to dieback disease identified using Associative Transcriptomics. Scientific Reports, 6, Article 19335. https://doi.org/10.1038/srep19335

Harper, AL, McKinney, LV, Nielsen, LR, Havlickova, L, Li, Y, Trick, M, Fraser, F, Wang, L, Fellgett, A, Sollars, ESA, Janacek, SH, Downie, JA, Buggs, RJA, Kjær, ED & Bancroft, I 2016, 'Molecular markers for tolerance of European ash (Fraxinus excelsior) to dieback disease identified using Associative Transcriptomics', Scientific Reports, vol. 6, 19335. https://doi.org/10.1038/srep19335

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title = "Molecular markers for tolerance of European ash (Fraxinus excelsior) to dieback disease identified using Associative Transcriptomics",

abstract = "Tree disease epidemics are a global problem, impacting food security, biodiversity and national economies. The potential for conservation and breeding in trees is hampered by complex genomes and long lifecycles, with most species lacking genomic resources. The European Ash tree Fraxinus excelsior is being devastated by the fungal pathogen Hymenoscyphus fraxineus, which causes ash dieback disease. Taking this system as an example and utilizing Associative Transcriptomics for the first time in a plant pathology study, we discovered gene sequence and gene expression variants across a genetic diversity panel scored for disease symptoms and identified markers strongly associated with canopy damage in infected trees. Using these markers we predicted phenotypes in a test panel of unrelated trees, successfully identifying individuals with a low level of susceptibility to the disease. Co-expression analysis suggested that pre-priming of defence responses may underlie reduced susceptibility to ash dieback.",

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AU - Nielsen, Lene Rostgaard

AU - Havlickova, Lenka

AU - Li, Yi

AU - Trick, Martin

AU - Fraser, Fiona

AU - Wang, Lihong

AU - Fellgett, Alison

AU - Sollars, Elizabeth S A

AU - Janacek, Sophie H.

AU - Downie, J. Allan

AU - Buggs, Richard J A

AU - Kjær, Erik Dahl

AU - Bancroft, Ian

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N2 - Tree disease epidemics are a global problem, impacting food security, biodiversity and national economies. The potential for conservation and breeding in trees is hampered by complex genomes and long lifecycles, with most species lacking genomic resources. The European Ash tree Fraxinus excelsior is being devastated by the fungal pathogen Hymenoscyphus fraxineus, which causes ash dieback disease. Taking this system as an example and utilizing Associative Transcriptomics for the first time in a plant pathology study, we discovered gene sequence and gene expression variants across a genetic diversity panel scored for disease symptoms and identified markers strongly associated with canopy damage in infected trees. Using these markers we predicted phenotypes in a test panel of unrelated trees, successfully identifying individuals with a low level of susceptibility to the disease. Co-expression analysis suggested that pre-priming of defence responses may underlie reduced susceptibility to ash dieback.

AB - Tree disease epidemics are a global problem, impacting food security, biodiversity and national economies. The potential for conservation and breeding in trees is hampered by complex genomes and long lifecycles, with most species lacking genomic resources. The European Ash tree Fraxinus excelsior is being devastated by the fungal pathogen Hymenoscyphus fraxineus, which causes ash dieback disease. Taking this system as an example and utilizing Associative Transcriptomics for the first time in a plant pathology study, we discovered gene sequence and gene expression variants across a genetic diversity panel scored for disease symptoms and identified markers strongly associated with canopy damage in infected trees. Using these markers we predicted phenotypes in a test panel of unrelated trees, successfully identifying individuals with a low level of susceptibility to the disease. Co-expression analysis suggested that pre-priming of defence responses may underlie reduced susceptibility to ash dieback.

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Molecular markers for tolerance of European ash (Fraxinus excelsior) to dieback disease identified using Associative Transcriptomics

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