TY - JOUR
T1 - Molecular identification of a Drosophila G protein-coupled receptor specific for crustacean cardioactive peptide.
AU - Cazzamali, Giuseppe
AU - Hauser, Frank
AU - Kobberup, Sune
AU - Williamson, Michael
AU - Grimmelikhuijzen, Cornelis J P
N1 - Keywords: Amino Acid Sequence; Animals; Anopheles; Base Sequence; Blotting, Northern; CHO Cells; Cricetinae; DNA; DNA, Complementary; Databases as Topic; Drosophila; GTP-Binding Proteins; Molecular Sequence Data; Neuropeptides; Receptors, Cell Surface; Sequence Homology, Amino Acid; Tissue Distribution; Transfection
PY - 2003
Y1 - 2003
N2 - The Drosophila Genome Project website (www.flybase.org) contains the sequence of an annotated gene (CG6111) expected to code for a G protein-coupled receptor. We have cloned this receptor and found that its gene was not correctly predicted, because an annotated neighbouring gene (CG14547) was also part of the receptor gene. DNA corresponding to the corrected gene CG6111 was expressed in Chinese hamster ovary cells, where it was found to code for a receptor that could be activated by low concentrations of crustacean cardioactive peptide, which is a neuropeptide also known to occur in Drosophila and other insects (EC(50), 5.4 x 10(-10)M). Other known Drosophila neuropeptides, such as adipokinetic hormone, did not activate the receptor. The receptor is expressed in all developmental stages from Drosophila, but only very weakly in larvae. In adult flies, the receptor is mainly expressed in the head. Furthermore, we identified a gene sequence in the genomic database from the malaria mosquito Anopheles gambiae that very likely codes for a crustacean cardioactive peptide receptor.
AB - The Drosophila Genome Project website (www.flybase.org) contains the sequence of an annotated gene (CG6111) expected to code for a G protein-coupled receptor. We have cloned this receptor and found that its gene was not correctly predicted, because an annotated neighbouring gene (CG14547) was also part of the receptor gene. DNA corresponding to the corrected gene CG6111 was expressed in Chinese hamster ovary cells, where it was found to code for a receptor that could be activated by low concentrations of crustacean cardioactive peptide, which is a neuropeptide also known to occur in Drosophila and other insects (EC(50), 5.4 x 10(-10)M). Other known Drosophila neuropeptides, such as adipokinetic hormone, did not activate the receptor. The receptor is expressed in all developmental stages from Drosophila, but only very weakly in larvae. In adult flies, the receptor is mainly expressed in the head. Furthermore, we identified a gene sequence in the genomic database from the malaria mosquito Anopheles gambiae that very likely codes for a crustacean cardioactive peptide receptor.
U2 - 10.1016/S0006-291X(03)00302-4
DO - 10.1016/S0006-291X(03)00302-4
M3 - Journal article
C2 - 12646179
SN - 0006-291X
VL - 303
SP - 146
EP - 152
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -
