Abstract
Using oligonucleotide probes derived from consensus sequences of known vertebrate and invertebrate G protein-coupled receptors, we have cloned the cDNA for a presumed G protein-coupled receptor from sea anemones. This receptor shows a striking structural homology with members of the glycoprotein hormone (FSH, TSH, LH/CG) receptor family from mammals, including a very large, extracellular N terminus (18-25% sequence identity) and a 7 transmembrane region (44-48% sequence identity). As with the mammalian glycoprotein hormone receptor genes, the sea anemone receptor gene yields transcripts which can be alternatively spliced, thereby yielding a shortened receptor variant only containing the large extracellular (soluble) N terminus. All this is strong evidence that the putative glycoprotein hormone receptor from sea anemones is evolutionarily related to those from mammals. This is the first report showing that a putative glycoprotein hormone receptor occurs in invertebrates.
| Original language | English |
|---|---|
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 197 |
| Issue number | 3 |
| Pages (from-to) | 1062-9 |
| Number of pages | 8 |
| ISSN | 0006-291X |
| DOIs | |
| Publication status | Published - 30 Dec 1993 |
Keywords
- Amino Acid Sequence
- Animals
- Base Sequence
- Cloning, Molecular
- DNA Primers
- Exons
- GTP-Binding Proteins
- Gene Library
- Mammals
- Molecular Sequence Data
- Oligonucleotide Probes
- Polymerase Chain Reaction
- Rats
- Receptors, Cell Surface
- Receptors, FSH
- Receptors, LH
- Receptors, Thyrotropin
- Repetitive Sequences, Nucleic Acid
- Sea Anemones
- Sequence Homology, Amino Acid