Abstract
Using oligonucleotide probes derived from consensus sequences of known vertebrate and invertebrate G protein-coupled receptors, we have cloned the cDNA for a presumed G protein-coupled receptor from sea anemones. This receptor shows a striking structural homology with members of the glycoprotein hormone (FSH, TSH, LH/CG) receptor family from mammals, including a very large, extracellular N terminus (18-25% sequence identity) and a 7 transmembrane region (44-48% sequence identity). As with the mammalian glycoprotein hormone receptor genes, the sea anemone receptor gene yields transcripts which can be alternatively spliced, thereby yielding a shortened receptor variant only containing the large extracellular (soluble) N terminus. All this is strong evidence that the putative glycoprotein hormone receptor from sea anemones is evolutionarily related to those from mammals. This is the first report showing that a putative glycoprotein hormone receptor occurs in invertebrates.
| Originalsprog | Engelsk |
|---|---|
| Tidsskrift | Biochemical and Biophysical Research Communications |
| Vol/bind | 197 |
| Udgave nummer | 3 |
| Sider (fra-til) | 1062-9 |
| Antal sider | 8 |
| ISSN | 0006-291X |
| DOI | |
| Status | Udgivet - 30 dec. 1993 |