Molecular cloning and functional expression of the first two specific insect myosuppressin receptors.

Kristoffer Egerod; Eyjólfur Reynisson; Frank Hauser; Giuseppe Cazzamali; Michael Williamson; Cornelis J P Grimmelikhuijzen

doi:10.1073/pnas.1632197100

Molecular cloning and functional expression of the first two specific insect myosuppressin receptors.

Kristoffer Egerod, Eyjólfur Reynisson, Frank Hauser, Giuseppe Cazzamali, Michael Williamson, Cornelis J P Grimmelikhuijzen

Cell and Neurobiology

75 Citations (Scopus)

Abstract

The Drosophila Genome Project database contains the sequences of two genes, CG8985 and CG13803, which are predicted to code for G protein-coupled receptors. We cloned the cDNAs corresponding to these genes and found that their gene structures had not been correctly annotated. We subsequently expressed the coding regions of the two corrected receptor genes in Chinese hamster ovary cells and found that each of them coded for a receptor that could be activated by low concentrations of Drosophila myosuppressin (EC50,4 x 10(-8) M). The insect myosuppressins are decapeptides that generally inhibit insect visceral muscles. Other tested Drosophila neuropeptides did not activate the two receptors. In addition to the two Drosophila myosuppressin receptors, we identified a sequence in the genomic database from the malaria mosquito Anopheles gambiae that also very likely codes for a myosuppressin receptor. To our knowledge, this paper is the first report on the molecular identification of specific insect myosuppressin receptors.

Original language	English
Journal	Proceedings of the National Academy of Science of the United States of America
Volume	100
Issue number	17
Pages (from-to)	9808-13
Number of pages	5
ISSN	0027-8424
DOIs	https://doi.org/10.1073/pnas.1632197100
Publication status	Published - 2003

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Molecular cloning and functional expression of the first two specific insect myosuppressin receptors. / Egerod, Kristoffer; Reynisson, Eyjólfur; Hauser, Frank et al.
In: Proceedings of the National Academy of Science of the United States of America, Vol. 100, No. 17, 2003, p. 9808-13.

Research output: Contribution to journal › Journal article › Research › peer-review

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title = "Molecular cloning and functional expression of the first two specific insect myosuppressin receptors.",

abstract = "The Drosophila Genome Project database contains the sequences of two genes, CG8985 and CG13803, which are predicted to code for G protein-coupled receptors. We cloned the cDNAs corresponding to these genes and found that their gene structures had not been correctly annotated. We subsequently expressed the coding regions of the two corrected receptor genes in Chinese hamster ovary cells and found that each of them coded for a receptor that could be activated by low concentrations of Drosophila myosuppressin (EC50,4 x 10(-8) M). The insect myosuppressins are decapeptides that generally inhibit insect visceral muscles. Other tested Drosophila neuropeptides did not activate the two receptors. In addition to the two Drosophila myosuppressin receptors, we identified a sequence in the genomic database from the malaria mosquito Anopheles gambiae that also very likely codes for a myosuppressin receptor. To our knowledge, this paper is the first report on the molecular identification of specific insect myosuppressin receptors.",

author = "Kristoffer Egerod and Eyj{\'o}lfur Reynisson and Frank Hauser and Giuseppe Cazzamali and Michael Williamson and Grimmelikhuijzen, {Cornelis J P}",

note = "Keywords: Amino Acid Sequence; Animals; Base Sequence; CHO Cells; Cell Line; Cloning, Molecular; Cricetinae; DNA, Complementary; Drosophila Proteins; Drosophila melanogaster; Exons; GTP-Binding Proteins; Gene Expression; Genes, Insect; Introns; Molecular Sequence Data; Neuropeptides; Receptors, Cell Surface; Receptors, Peptide; Sequence Homology, Amino Acid",

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AU - Cazzamali, Giuseppe

AU - Williamson, Michael

AU - Grimmelikhuijzen, Cornelis J P

N1 - Keywords: Amino Acid Sequence; Animals; Base Sequence; CHO Cells; Cell Line; Cloning, Molecular; Cricetinae; DNA, Complementary; Drosophila Proteins; Drosophila melanogaster; Exons; GTP-Binding Proteins; Gene Expression; Genes, Insect; Introns; Molecular Sequence Data; Neuropeptides; Receptors, Cell Surface; Receptors, Peptide; Sequence Homology, Amino Acid

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N2 - The Drosophila Genome Project database contains the sequences of two genes, CG8985 and CG13803, which are predicted to code for G protein-coupled receptors. We cloned the cDNAs corresponding to these genes and found that their gene structures had not been correctly annotated. We subsequently expressed the coding regions of the two corrected receptor genes in Chinese hamster ovary cells and found that each of them coded for a receptor that could be activated by low concentrations of Drosophila myosuppressin (EC50,4 x 10(-8) M). The insect myosuppressins are decapeptides that generally inhibit insect visceral muscles. Other tested Drosophila neuropeptides did not activate the two receptors. In addition to the two Drosophila myosuppressin receptors, we identified a sequence in the genomic database from the malaria mosquito Anopheles gambiae that also very likely codes for a myosuppressin receptor. To our knowledge, this paper is the first report on the molecular identification of specific insect myosuppressin receptors.

AB - The Drosophila Genome Project database contains the sequences of two genes, CG8985 and CG13803, which are predicted to code for G protein-coupled receptors. We cloned the cDNAs corresponding to these genes and found that their gene structures had not been correctly annotated. We subsequently expressed the coding regions of the two corrected receptor genes in Chinese hamster ovary cells and found that each of them coded for a receptor that could be activated by low concentrations of Drosophila myosuppressin (EC50,4 x 10(-8) M). The insect myosuppressins are decapeptides that generally inhibit insect visceral muscles. Other tested Drosophila neuropeptides did not activate the two receptors. In addition to the two Drosophila myosuppressin receptors, we identified a sequence in the genomic database from the malaria mosquito Anopheles gambiae that also very likely codes for a myosuppressin receptor. To our knowledge, this paper is the first report on the molecular identification of specific insect myosuppressin receptors.

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Molecular cloning and functional expression of the first two specific insect myosuppressin receptors.

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