Molecular characterization of SAT 2 foot-and-mouth disease virus from post-outbreak slaughtered animals: implications for disease control in Uganda

Sheila Balinda; Graham Belsham; C Masembe; Abraham K. Sangula; H R Siegismund; V B Muwanika

doi:10.1017/s0950268809991427

Molecular characterization of SAT 2 foot-and-mouth disease virus from post-outbreak slaughtered animals: implications for disease control in Uganda

Sheila Balinda, Graham Belsham, C Masembe, Abraham K. Sangula, H R Siegismund, V B Muwanika

Computational and RNA Biology

13 Citations (Scopus)

Abstract

In Uganda, limiting the extent of foot-and-mouth disease (FMD) spread during outbreaks involves short-term measures such as ring vaccination and restrictions of the movement of livestock and their products to and from the affected areas. In this study, the presence of FMD virus RNA was investigated in cattle samples 3 months after FMD quarantine measures had been lifted following an outbreak in 2004. Oropharyngeal tissue samples were obtained from 12 cattle slaughtered in a small town abattoir in Kiboga. FMD virus RNA was detected by diagnostic RT-PCR in nine of the 12 tissue samples. Part of the coding region for the capsid protein VP1 was amplified and sequenced. All samples were identified as belonging to the SAT 2 serotype. The implications for FMD control of both virus introduction into Uganda and the presence of carrier animals following outbreaks are discussed.

Original language	English
Journal	Epidemiology and Infection
Volume	138
Issue number	8
Pages (from-to)	1204-10
Number of pages	7
ISSN	0950-2688
DOIs	https://doi.org/10.1017/s0950268809991427
Publication status	Published - 1 Aug 2010

Keywords

Animals
Base Composition
Base Sequence
Capsid Proteins
Cattle
Disease Outbreaks
Foot-and-Mouth Disease
Foot-and-Mouth Disease Virus
Molecular Sequence Data
Phylogeny
Quarantine
RNA, Viral
Reverse Transcriptase Polymerase Chain Reaction
Sequence Alignment
Uganda

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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Cite this

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title = "Molecular characterization of SAT 2 foot-and-mouth disease virus from post-outbreak slaughtered animals: implications for disease control in Uganda",

abstract = "In Uganda, limiting the extent of foot-and-mouth disease (FMD) spread during outbreaks involves short-term measures such as ring vaccination and restrictions of the movement of livestock and their products to and from the affected areas. In this study, the presence of FMD virus RNA was investigated in cattle samples 3 months after FMD quarantine measures had been lifted following an outbreak in 2004. Oropharyngeal tissue samples were obtained from 12 cattle slaughtered in a small town abattoir in Kiboga. FMD virus RNA was detected by diagnostic RT-PCR in nine of the 12 tissue samples. Part of the coding region for the capsid protein VP1 was amplified and sequenced. All samples were identified as belonging to the SAT 2 serotype. The implications for FMD control of both virus introduction into Uganda and the presence of carrier animals following outbreaks are discussed.",

keywords = "Animals, Base Composition, Base Sequence, Capsid Proteins, Cattle, Disease Outbreaks, Foot-and-Mouth Disease, Foot-and-Mouth Disease Virus, Molecular Sequence Data, Phylogeny, Quarantine, RNA, Viral, Reverse Transcriptase Polymerase Chain Reaction, Sequence Alignment, Uganda",

author = "Sheila Balinda and Graham Belsham and C Masembe and Sangula, {Abraham K.} and Siegismund, {H R} and Muwanika, {V B}",

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doi = "10.1017/s0950268809991427",

language = "English",

volume = "138",

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journal = "Epidemiology and Infection",

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T1 - Molecular characterization of SAT 2 foot-and-mouth disease virus from post-outbreak slaughtered animals: implications for disease control in Uganda

AU - Balinda, Sheila

AU - Belsham, Graham

AU - Masembe, C

AU - Sangula, Abraham K.

AU - Siegismund, H R

AU - Muwanika, V B

PY - 2010/8/1

Y1 - 2010/8/1

N2 - In Uganda, limiting the extent of foot-and-mouth disease (FMD) spread during outbreaks involves short-term measures such as ring vaccination and restrictions of the movement of livestock and their products to and from the affected areas. In this study, the presence of FMD virus RNA was investigated in cattle samples 3 months after FMD quarantine measures had been lifted following an outbreak in 2004. Oropharyngeal tissue samples were obtained from 12 cattle slaughtered in a small town abattoir in Kiboga. FMD virus RNA was detected by diagnostic RT-PCR in nine of the 12 tissue samples. Part of the coding region for the capsid protein VP1 was amplified and sequenced. All samples were identified as belonging to the SAT 2 serotype. The implications for FMD control of both virus introduction into Uganda and the presence of carrier animals following outbreaks are discussed.

AB - In Uganda, limiting the extent of foot-and-mouth disease (FMD) spread during outbreaks involves short-term measures such as ring vaccination and restrictions of the movement of livestock and their products to and from the affected areas. In this study, the presence of FMD virus RNA was investigated in cattle samples 3 months after FMD quarantine measures had been lifted following an outbreak in 2004. Oropharyngeal tissue samples were obtained from 12 cattle slaughtered in a small town abattoir in Kiboga. FMD virus RNA was detected by diagnostic RT-PCR in nine of the 12 tissue samples. Part of the coding region for the capsid protein VP1 was amplified and sequenced. All samples were identified as belonging to the SAT 2 serotype. The implications for FMD control of both virus introduction into Uganda and the presence of carrier animals following outbreaks are discussed.

KW - Animals

KW - Base Composition

KW - Base Sequence

KW - Capsid Proteins

KW - Cattle

KW - Disease Outbreaks

KW - Foot-and-Mouth Disease

KW - Foot-and-Mouth Disease Virus

KW - Molecular Sequence Data

KW - Phylogeny

KW - Quarantine

KW - RNA, Viral

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - Sequence Alignment

KW - Uganda

U2 - 10.1017/s0950268809991427

DO - 10.1017/s0950268809991427

M3 - Journal article

C2 - 20003615

SN - 0950-2688

VL - 138

SP - 1204

EP - 1210

JO - Epidemiology and Infection

JF - Epidemiology and Infection

IS - 8

ER -

Molecular characterization of SAT 2 foot-and-mouth disease virus from post-outbreak slaughtered animals: implications for disease control in Uganda

Abstract

Keywords

UN SDGs

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