Molecular characterization of SAT 2 foot-and-mouth disease virus from post-outbreak slaughtered animals: implications for disease control in Uganda

TY - JOUR

T1 - Molecular characterization of SAT 2 foot-and-mouth disease virus from post-outbreak slaughtered animals: implications for disease control in Uganda

AU - Balinda, Sheila

AU - Belsham, Graham

AU - Masembe, C

AU - Sangula, Abraham K.

AU - Siegismund, H R

AU - Muwanika, V B

PY - 2010/8/1

Y1 - 2010/8/1

N2 - In Uganda, limiting the extent of foot-and-mouth disease (FMD) spread during outbreaks involves short-term measures such as ring vaccination and restrictions of the movement of livestock and their products to and from the affected areas. In this study, the presence of FMD virus RNA was investigated in cattle samples 3 months after FMD quarantine measures had been lifted following an outbreak in 2004. Oropharyngeal tissue samples were obtained from 12 cattle slaughtered in a small town abattoir in Kiboga. FMD virus RNA was detected by diagnostic RT-PCR in nine of the 12 tissue samples. Part of the coding region for the capsid protein VP1 was amplified and sequenced. All samples were identified as belonging to the SAT 2 serotype. The implications for FMD control of both virus introduction into Uganda and the presence of carrier animals following outbreaks are discussed.

AB - In Uganda, limiting the extent of foot-and-mouth disease (FMD) spread during outbreaks involves short-term measures such as ring vaccination and restrictions of the movement of livestock and their products to and from the affected areas. In this study, the presence of FMD virus RNA was investigated in cattle samples 3 months after FMD quarantine measures had been lifted following an outbreak in 2004. Oropharyngeal tissue samples were obtained from 12 cattle slaughtered in a small town abattoir in Kiboga. FMD virus RNA was detected by diagnostic RT-PCR in nine of the 12 tissue samples. Part of the coding region for the capsid protein VP1 was amplified and sequenced. All samples were identified as belonging to the SAT 2 serotype. The implications for FMD control of both virus introduction into Uganda and the presence of carrier animals following outbreaks are discussed.

KW - Animals

KW - Base Composition

KW - Base Sequence

KW - Capsid Proteins

KW - Cattle

KW - Disease Outbreaks

KW - Foot-and-Mouth Disease

KW - Foot-and-Mouth Disease Virus

KW - Molecular Sequence Data

KW - Phylogeny

KW - Quarantine

KW - RNA, Viral

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - Sequence Alignment

KW - Uganda

U2 - 10.1017/s0950268809991427

DO - 10.1017/s0950268809991427

M3 - Journal article

C2 - 20003615

SN - 0950-2688

VL - 138

SP - 1204

EP - 1210

JO - Epidemiology and Infection

JF - Epidemiology and Infection

IS - 8

ER -