Molecular and epidemiological profiles of hepatitis C virus genotype 4 in Denmark

Mette Brandt Eriksen; Louise Bruun Jørgensen; Henrik Krarup; Alex Lund Laursen; Peer Brehm Christensen; Axel Møller; Poul Schlichting; Carla Kuiken; Jens Bukh; Nina Weis; DANHEP Group

doi:10.1002/jmv.21896

Molecular and epidemiological profiles of hepatitis C virus genotype 4 in Denmark

Mette Brandt Eriksen, Louise Bruun Jørgensen, Henrik Krarup, Alex Lund Laursen, Peer Brehm Christensen, Axel Møller, Poul Schlichting, Carla Kuiken, Jens Bukh, Nina Weis, DANHEP Group

Department of Immunology and Microbiology

9 Citations (Scopus)

Abstract

The prevalence of hepatitis C virus (HCV) genotype 4 has increased throughout Europe. This is an epidemiological study of patients infected chronically with HCV genotype 4 in Denmark. The HCV strains analyzed originated from patient samples collected between 1999 and 2007 as part of the national Danish hepatitis B and C network, DANHEP. Sequence analyses were based on the envelope 1 region of HCV. Results from a total of 72 patients indicated a high degree of genetic heterogeneity. Fifty-six patients (78%) were infected with one of the three dominating subtypes: 4d, 4a, or 4r. The remaining 16 patients (22%) were infected with subtypes 4h, 4k, 4l, 4n, 4o, or 4Unclassified. Three epidemiological profiles were identified: (1) patients infected with HCV by intravenous drug use were infected solely with subtype 4d. They were all of European origin, and 15 of the 16 patients were ethnic Danes. No single transmission event could be confirmed, but the pairwise nucleotide identity within the patients of Danish origin was relatively high (~95%), suggesting a recent introduction into Denmark. (2) The 21 patients infected with subtype 4a all came from Northern Africa, Egypt, Pakistan, or the Middle East. (3) Patients from Southern Africa dominated among patients infected with subtype 4r (10 of 12 patients). This study demonstrates that HCV genotype 4d has been introduced in and spread among Danish intravenous drug users. The remaining subtypes show restricted distribution, infecting almost exclusively patients from geographical areas with a relatively high prevalence of HCV genotype 4 infections. J. Med. Virol. 82:1869-1877, 2010.

Original language	English
Journal	Journal of Medical Virology
Volume	82
Issue number	11
Pages (from-to)	1869-1877
Number of pages	9
ISSN	0146-6615
DOIs	https://doi.org/10.1002/jmv.21896
Publication status	Published - Nov 2010

Keywords

Adult
Aged
Denmark
Female
Genotype
HIV Infections
Hepacivirus
Hepatitis C, Chronic
Humans
Male
Middle Aged
Molecular Epidemiology
Phylogeny
Prevalence
Sequence Analysis, DNA
Substance Abuse, Intravenous
Viral Envelope Proteins

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1002/jmv.21896

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title = "Molecular and epidemiological profiles of hepatitis C virus genotype 4 in Denmark",

abstract = "The prevalence of hepatitis C virus (HCV) genotype 4 has increased throughout Europe. This is an epidemiological study of patients infected chronically with HCV genotype 4 in Denmark. The HCV strains analyzed originated from patient samples collected between 1999 and 2007 as part of the national Danish hepatitis B and C network, DANHEP. Sequence analyses were based on the envelope 1 region of HCV. Results from a total of 72 patients indicated a high degree of genetic heterogeneity. Fifty-six patients (78%) were infected with one of the three dominating subtypes: 4d, 4a, or 4r. The remaining 16 patients (22%) were infected with subtypes 4h, 4k, 4l, 4n, 4o, or 4Unclassified. Three epidemiological profiles were identified: (1) patients infected with HCV by intravenous drug use were infected solely with subtype 4d. They were all of European origin, and 15 of the 16 patients were ethnic Danes. No single transmission event could be confirmed, but the pairwise nucleotide identity within the patients of Danish origin was relatively high (~95%), suggesting a recent introduction into Denmark. (2) The 21 patients infected with subtype 4a all came from Northern Africa, Egypt, Pakistan, or the Middle East. (3) Patients from Southern Africa dominated among patients infected with subtype 4r (10 of 12 patients). This study demonstrates that HCV genotype 4d has been introduced in and spread among Danish intravenous drug users. The remaining subtypes show restricted distribution, infecting almost exclusively patients from geographical areas with a relatively high prevalence of HCV genotype 4 infections. J. Med. Virol. 82:1869-1877, 2010.",

keywords = "Adult, Aged, Denmark, Female, Genotype, HIV Infections, Hepacivirus, Hepatitis C, Chronic, Humans, Male, Middle Aged, Molecular Epidemiology, Phylogeny, Prevalence, Sequence Analysis, DNA, Substance Abuse, Intravenous, Viral Envelope Proteins",

author = "Eriksen, {Mette Brandt} and J{\o}rgensen, {Louise Bruun} and Henrik Krarup and Laursen, {Alex Lund} and Christensen, {Peer Brehm} and Axel M{\o}ller and Poul Schlichting and Carla Kuiken and Jens Bukh and Nina Weis and {DANHEP Group}",

note = "{\textcopyright} 2010 Wiley-Liss, Inc.",

year = "2010",

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TY - JOUR

T1 - Molecular and epidemiological profiles of hepatitis C virus genotype 4 in Denmark

AU - Eriksen, Mette Brandt

AU - Jørgensen, Louise Bruun

AU - Krarup, Henrik

AU - Laursen, Alex Lund

AU - Christensen, Peer Brehm

AU - Møller, Axel

AU - Schlichting, Poul

AU - Kuiken, Carla

AU - Bukh, Jens

AU - Weis, Nina

AU - DANHEP Group

PY - 2010/11

Y1 - 2010/11

N2 - The prevalence of hepatitis C virus (HCV) genotype 4 has increased throughout Europe. This is an epidemiological study of patients infected chronically with HCV genotype 4 in Denmark. The HCV strains analyzed originated from patient samples collected between 1999 and 2007 as part of the national Danish hepatitis B and C network, DANHEP. Sequence analyses were based on the envelope 1 region of HCV. Results from a total of 72 patients indicated a high degree of genetic heterogeneity. Fifty-six patients (78%) were infected with one of the three dominating subtypes: 4d, 4a, or 4r. The remaining 16 patients (22%) were infected with subtypes 4h, 4k, 4l, 4n, 4o, or 4Unclassified. Three epidemiological profiles were identified: (1) patients infected with HCV by intravenous drug use were infected solely with subtype 4d. They were all of European origin, and 15 of the 16 patients were ethnic Danes. No single transmission event could be confirmed, but the pairwise nucleotide identity within the patients of Danish origin was relatively high (~95%), suggesting a recent introduction into Denmark. (2) The 21 patients infected with subtype 4a all came from Northern Africa, Egypt, Pakistan, or the Middle East. (3) Patients from Southern Africa dominated among patients infected with subtype 4r (10 of 12 patients). This study demonstrates that HCV genotype 4d has been introduced in and spread among Danish intravenous drug users. The remaining subtypes show restricted distribution, infecting almost exclusively patients from geographical areas with a relatively high prevalence of HCV genotype 4 infections. J. Med. Virol. 82:1869-1877, 2010.

AB - The prevalence of hepatitis C virus (HCV) genotype 4 has increased throughout Europe. This is an epidemiological study of patients infected chronically with HCV genotype 4 in Denmark. The HCV strains analyzed originated from patient samples collected between 1999 and 2007 as part of the national Danish hepatitis B and C network, DANHEP. Sequence analyses were based on the envelope 1 region of HCV. Results from a total of 72 patients indicated a high degree of genetic heterogeneity. Fifty-six patients (78%) were infected with one of the three dominating subtypes: 4d, 4a, or 4r. The remaining 16 patients (22%) were infected with subtypes 4h, 4k, 4l, 4n, 4o, or 4Unclassified. Three epidemiological profiles were identified: (1) patients infected with HCV by intravenous drug use were infected solely with subtype 4d. They were all of European origin, and 15 of the 16 patients were ethnic Danes. No single transmission event could be confirmed, but the pairwise nucleotide identity within the patients of Danish origin was relatively high (~95%), suggesting a recent introduction into Denmark. (2) The 21 patients infected with subtype 4a all came from Northern Africa, Egypt, Pakistan, or the Middle East. (3) Patients from Southern Africa dominated among patients infected with subtype 4r (10 of 12 patients). This study demonstrates that HCV genotype 4d has been introduced in and spread among Danish intravenous drug users. The remaining subtypes show restricted distribution, infecting almost exclusively patients from geographical areas with a relatively high prevalence of HCV genotype 4 infections. J. Med. Virol. 82:1869-1877, 2010.

KW - Adult

KW - Aged

KW - Denmark

KW - Female

KW - Genotype

KW - HIV Infections

KW - Hepacivirus

KW - Hepatitis C, Chronic

KW - Humans

KW - Male

KW - Middle Aged

KW - Molecular Epidemiology

KW - Phylogeny

KW - Prevalence

KW - Sequence Analysis, DNA

KW - Substance Abuse, Intravenous

KW - Viral Envelope Proteins

U2 - 10.1002/jmv.21896

DO - 10.1002/jmv.21896

M3 - Journal article

C2 - 20872713

SN - 0146-6615

VL - 82

SP - 1869

EP - 1877

JO - Journal of Medical Virology

JF - Journal of Medical Virology

IS - 11

ER -

Molecular and epidemiological profiles of hepatitis C virus genotype 4 in Denmark

Abstract

Keywords

UN SDGs

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