Abstract
Despite the biological importance and pharmacological potential of glycans from marine organisms, there are many unanswered questions regarding their distribution, function, and evolution. Here we describe microarray-based glycan profiling of a diverse selection of marine animals using antibodies raised against fucoidan isolated from a brown alga. We demonstrate the presence of two fucoidan epitopes in six animals belonging to three phyla including Porifera, Molusca, and Chordata. We studied the spatial distribution of these epitopes in Cliona celata ("boring sponge") and identified their restricted localization on the surface of internal chambers. Our results show the potential of high-throughput screening and probes commonly used in plant and algal cell wall biology to study the diversity and distribution of glycan structures in metazoans.
| Original language | English |
|---|---|
| Article number | 293 |
| Journal | Frontiers in Marine Science |
| Volume | 4 |
| Number of pages | 8 |
| DOIs | |
| Publication status | Published - 2017 |
Keywords
- Anti-glycan antibodies
- Cliona celata
- Fucoidans
- Immunolocalisation
- Marine fauna
- Microarray profiling
- Sponges
- Structural polysaccharides
