Microarray glycan profiling reveals algal fucoidan epitopes in diverse marine metazoans

TY - JOUR

T1 - Microarray glycan profiling reveals algal fucoidan epitopes in diverse marine metazoans

AU - Asunción Salmeán, Armando

AU - Hervé, Cécile

AU - Jørgensen, Bodil

AU - Willats, William George Tycho

AU - Mravec, Jozef

PY - 2017

Y1 - 2017

N2 - Despite the biological importance and pharmacological potential of glycans from marine organisms, there are many unanswered questions regarding their distribution, function, and evolution. Here we describe microarray-based glycan profiling of a diverse selection of marine animals using antibodies raised against fucoidan isolated from a brown alga. We demonstrate the presence of two fucoidan epitopes in six animals belonging to three phyla including Porifera, Molusca, and Chordata. We studied the spatial distribution of these epitopes in Cliona celata ("boring sponge") and identified their restricted localization on the surface of internal chambers. Our results show the potential of high-throughput screening and probes commonly used in plant and algal cell wall biology to study the diversity and distribution of glycan structures in metazoans.

AB - Despite the biological importance and pharmacological potential of glycans from marine organisms, there are many unanswered questions regarding their distribution, function, and evolution. Here we describe microarray-based glycan profiling of a diverse selection of marine animals using antibodies raised against fucoidan isolated from a brown alga. We demonstrate the presence of two fucoidan epitopes in six animals belonging to three phyla including Porifera, Molusca, and Chordata. We studied the spatial distribution of these epitopes in Cliona celata ("boring sponge") and identified their restricted localization on the surface of internal chambers. Our results show the potential of high-throughput screening and probes commonly used in plant and algal cell wall biology to study the diversity and distribution of glycan structures in metazoans.

KW - Anti-glycan antibodies

KW - Cliona celata

KW - Fucoidans

KW - Immunolocalisation

KW - Marine fauna

KW - Microarray profiling

KW - Sponges

KW - Structural polysaccharides

U2 - 10.3389/fmars.2017.00293

DO - 10.3389/fmars.2017.00293

M3 - Journal article

AN - SCOPUS:85029209941

SN - 2296-7745

VL - 4

JO - Frontiers in Marine Science

JF - Frontiers in Marine Science

M1 - 293

ER -