TY - JOUR
T1 - Methods for studying biofilm formation
T2 - flow cells and confocal laser scanning microscopy
AU - Tolker-Nielsen, Tim
AU - Sternberg, Claus
PY - 2014
Y1 - 2014
N2 - In this chapter methods for growing and analyzing biofilms under hydrodynamic conditions in flow cells are described. Use of flow cells allows for direct microscopic investigation of biofilm formation. The flow in these chambers is essentially laminar, which means that the biofilms can be grown under highly controlled conditions, and that perturbations such as addition of antibiotics or change of the growth medium can be done efficiently at a defined time point. The protocol includes construction of the flow cell and the bubble trap, assembly and sterilization of the flow cell system, inoculation of the flow cells, running of the system, confocal laser scanning microscopy and image analysis, and disassembly and cleaning of the system.
AB - In this chapter methods for growing and analyzing biofilms under hydrodynamic conditions in flow cells are described. Use of flow cells allows for direct microscopic investigation of biofilm formation. The flow in these chambers is essentially laminar, which means that the biofilms can be grown under highly controlled conditions, and that perturbations such as addition of antibiotics or change of the growth medium can be done efficiently at a defined time point. The protocol includes construction of the flow cell and the bubble trap, assembly and sterilization of the flow cell system, inoculation of the flow cells, running of the system, confocal laser scanning microscopy and image analysis, and disassembly and cleaning of the system.
U2 - 10.1007/978-1-4939-0473-0_47
DO - 10.1007/978-1-4939-0473-0_47
M3 - Journal article
C2 - 24818937
SN - 1064-3745
VL - 1149
SP - 615
EP - 629
JO - Methods in Molecular Biology
JF - Methods in Molecular Biology
ER -