TY - JOUR
T1 - Method for screening compounds that influence virulence gene expression in Staphylococcus aureus
AU - Nielsen, Anita
AU - Nielsen, Kristian Fog
AU - Frees, Dorte
AU - Larsen, Thomas Ostenfeld
AU - Ingmer, Hanne
PY - 2010/1
Y1 - 2010/1
N2 - We present a simple assay to examine effects of compounds on virulence gene expression in the human pathogen Staphylococcus aureus. The assay employs transcriptional reporter strains carrying lacZ fused to central virulence genes. Compounds affecting virulence gene expression and activity of the agr locus are scored based on color change in the presence of a chromogenic β-galactosidase substrate. The assay can be used to screen for novel antivirulence compounds from many different sources, such as fungi, as demonstrated here.
AB - We present a simple assay to examine effects of compounds on virulence gene expression in the human pathogen Staphylococcus aureus. The assay employs transcriptional reporter strains carrying lacZ fused to central virulence genes. Compounds affecting virulence gene expression and activity of the agr locus are scored based on color change in the presence of a chromogenic β-galactosidase substrate. The assay can be used to screen for novel antivirulence compounds from many different sources, such as fungi, as demonstrated here.
U2 - 10.1128/AAC.00940-09
DO - 10.1128/AAC.00940-09
M3 - Journal article
C2 - 19917763
SN - 0066-4804
VL - 54
SP - 509
EP - 512
JO - Antimicrobial Agents and Chemotherapy
JF - Antimicrobial Agents and Chemotherapy
IS - 1
ER -