Metabonomics in Ulcerative Colitis: Diagnostics, Biomarker Identification, And Insight into the Pathophysiology

Jacob T Bjerrum; Ole H Nielsen; Fuhua Hao; Huiru Tang; Jeremy K Nicholson; Yulan Wang; Jørgen Olsen; J.T. Bjerrum; O.H. Nielsen; Hao Shen; H.R. Tang; John Boyns Nicholson; Li Wang; J. Olsen

doi:10.1021/pr9008223

Metabonomics in Ulcerative Colitis: Diagnostics, Biomarker Identification, And Insight into the Pathophysiology

Jacob T Bjerrum, Ole H Nielsen, Fuhua Hao, Huiru Tang, Jeremy K Nicholson, Yulan Wang, Jørgen Olsen, J.T. Bjerrum, O.H. Nielsen, Hao Shen, H.R. Tang, John Boyns Nicholson, Li Wang, J. Olsen

118 Citations (Scopus)

Abstract

Nuclear magnetic resonance (NMR) spectroscopy and appropriate multivariate statistical analyses have been employed on mucosal colonic biopsies, colonocytes, lymphocytes, and urine from patients with ulcerative colitis (UC) and controls in order to explore the diagnostic possibilities, define new potential biomarkers, and generate a better understanding of the pathophysiology. Samples were collected from patients with active UC (n = 41), quiescent UC (n = 33), and from controls (n = 25) and analyzed by NMR spectroscopy. Data analysis was carried out by principal component analysis and orthogonal-projection to latent structure-discriminant analysis using the SIMCA P+11 software package (Umetrics, Umeå, Sweden) and Matlab environment. Significant differences between controls and active UC were discovered in the metabolic profiles of biopsies and colonocytes. In the biopsies from patients with active UC higher levels of antioxidants and of a range of amino acids, but lower levels of lipid, glycerophosphocholine (GPC), myo-inositol, and betaine were found, whereas the colonocytes only displayed low levels of GPC, myo-inositol and choline. Interestingly, 20% of inactive UC patients had similar profiles to those who were in an active state. This study demonstrates the possibilities of metabonomics as a diagnostic tool in active and quiescent UC and provides new insight into pathophysiologic mechanisms.

Original language	English
Journal	Journal of Proteome Research
Volume	9
Issue number	2
Pages (from-to)	954-962
Number of pages	9
ISSN	1535-3893
DOIs	https://doi.org/10.1021/pr9008223
Publication status	Published - 5 Feb 2010

Access to Document

10.1021/pr9008223

Cite this

Bjerrum, J. T., Nielsen, O. H., Hao, F., Tang, H., Nicholson, J. K., Wang, Y., Olsen, J., Bjerrum, J. T., Nielsen, O. H., Shen, H., Tang, H. R., Nicholson, J. B., Wang, L., & Olsen, J. (2010). Metabonomics in Ulcerative Colitis: Diagnostics, Biomarker Identification, And Insight into the Pathophysiology. Journal of Proteome Research, 9(2), 954-962. https://doi.org/10.1021/pr9008223

@article{238b497038d311dfad7f000ea68e967b,

title = "Metabonomics in Ulcerative Colitis: Diagnostics, Biomarker Identification, And Insight into the Pathophysiology",

abstract = "Nuclear magnetic resonance (NMR) spectroscopy and appropriate multivariate statistical analyses have been employed on mucosal colonic biopsies, colonocytes, lymphocytes, and urine from patients with ulcerative colitis (UC) and controls in order to explore the diagnostic possibilities, define new potential biomarkers, and generate a better understanding of the pathophysiology. Samples were collected from patients with active UC (n = 41), quiescent UC (n = 33), and from controls (n = 25) and analyzed by NMR spectroscopy. Data analysis was carried out by principal component analysis and orthogonal-projection to latent structure-discriminant analysis using the SIMCA P+11 software package (Umetrics, Ume{\aa}, Sweden) and Matlab environment. Significant differences between controls and active UC were discovered in the metabolic profiles of biopsies and colonocytes. In the biopsies from patients with active UC higher levels of antioxidants and of a range of amino acids, but lower levels of lipid, glycerophosphocholine (GPC), myo-inositol, and betaine were found, whereas the colonocytes only displayed low levels of GPC, myo-inositol and choline. Interestingly, 20% of inactive UC patients had similar profiles to those who were in an active state. This study demonstrates the possibilities of metabonomics as a diagnostic tool in active and quiescent UC and provides new insight into pathophysiologic mechanisms.",

author = "Bjerrum, {Jacob T} and Nielsen, {Ole H} and Fuhua Hao and Huiru Tang and Nicholson, {Jeremy K} and Yulan Wang and J{\o}rgen Olsen and J.T. Bjerrum and O.H. Nielsen and Hao Shen and H.R. Tang and Nicholson, {John Boyns} and Li Wang and J. Olsen",

year = "2010",

month = feb,

day = "5",

doi = "10.1021/pr9008223",

language = "English",

volume = "9",

pages = "954--962",

journal = "Journal of Proteome Research",

issn = "1535-3893",

publisher = "American Chemical Society",

number = "2",

}

TY - JOUR

T1 - Metabonomics in Ulcerative Colitis: Diagnostics, Biomarker Identification, And Insight into the Pathophysiology

AU - Bjerrum, Jacob T

AU - Nielsen, Ole H

AU - Hao, Fuhua

AU - Tang, Huiru

AU - Nicholson, Jeremy K

AU - Wang, Yulan

AU - Olsen, Jørgen

AU - Bjerrum, J.T.

AU - Nielsen, O.H.

AU - Shen, Hao

AU - Tang, H.R.

AU - Nicholson, John Boyns

AU - Wang, Li

AU - Olsen, J.

PY - 2010/2/5

Y1 - 2010/2/5

N2 - Nuclear magnetic resonance (NMR) spectroscopy and appropriate multivariate statistical analyses have been employed on mucosal colonic biopsies, colonocytes, lymphocytes, and urine from patients with ulcerative colitis (UC) and controls in order to explore the diagnostic possibilities, define new potential biomarkers, and generate a better understanding of the pathophysiology. Samples were collected from patients with active UC (n = 41), quiescent UC (n = 33), and from controls (n = 25) and analyzed by NMR spectroscopy. Data analysis was carried out by principal component analysis and orthogonal-projection to latent structure-discriminant analysis using the SIMCA P+11 software package (Umetrics, Umeå, Sweden) and Matlab environment. Significant differences between controls and active UC were discovered in the metabolic profiles of biopsies and colonocytes. In the biopsies from patients with active UC higher levels of antioxidants and of a range of amino acids, but lower levels of lipid, glycerophosphocholine (GPC), myo-inositol, and betaine were found, whereas the colonocytes only displayed low levels of GPC, myo-inositol and choline. Interestingly, 20% of inactive UC patients had similar profiles to those who were in an active state. This study demonstrates the possibilities of metabonomics as a diagnostic tool in active and quiescent UC and provides new insight into pathophysiologic mechanisms.

AB - Nuclear magnetic resonance (NMR) spectroscopy and appropriate multivariate statistical analyses have been employed on mucosal colonic biopsies, colonocytes, lymphocytes, and urine from patients with ulcerative colitis (UC) and controls in order to explore the diagnostic possibilities, define new potential biomarkers, and generate a better understanding of the pathophysiology. Samples were collected from patients with active UC (n = 41), quiescent UC (n = 33), and from controls (n = 25) and analyzed by NMR spectroscopy. Data analysis was carried out by principal component analysis and orthogonal-projection to latent structure-discriminant analysis using the SIMCA P+11 software package (Umetrics, Umeå, Sweden) and Matlab environment. Significant differences between controls and active UC were discovered in the metabolic profiles of biopsies and colonocytes. In the biopsies from patients with active UC higher levels of antioxidants and of a range of amino acids, but lower levels of lipid, glycerophosphocholine (GPC), myo-inositol, and betaine were found, whereas the colonocytes only displayed low levels of GPC, myo-inositol and choline. Interestingly, 20% of inactive UC patients had similar profiles to those who were in an active state. This study demonstrates the possibilities of metabonomics as a diagnostic tool in active and quiescent UC and provides new insight into pathophysiologic mechanisms.

U2 - 10.1021/pr9008223

DO - 10.1021/pr9008223

M3 - Journal article

C2 - 19860486

SN - 1535-3893

VL - 9

SP - 954

EP - 962

JO - Journal of Proteome Research

JF - Journal of Proteome Research

IS - 2

ER -

Metabonomics in Ulcerative Colitis: Diagnostics, Biomarker Identification, And Insight into the Pathophysiology

Abstract

Access to Document

Fingerprint

Cite this