Metabolism of polysaccharides in dynamic middle lamellae during cotton fibre development

Xiaoyuan Guo; Jean Luc Runavot; Stéphane Bourot; Frank Meulewaeter; Mercedes Hernandez-Gomez; Claire Holland; Jesper Harholt; William G.T. Willats; Jozef Mravec; Paul Knox; Peter Ulvskov

doi:10.1007/s00425-019-03107-4

Metabolism of polysaccharides in dynamic middle lamellae during cotton fibre development

Xiaoyuan Guo, Jean Luc Runavot, Stéphane Bourot, Frank Meulewaeter, Mercedes Hernandez-Gomez, Claire Holland, Jesper Harholt, William G.T. Willats, Jozef Mravec, Paul Knox, Peter Ulvskov^*

^*Corresponding author for this work

Section for Plant Glycobiology

4 Citations (Scopus)

Abstract

Main conclusion: Evidence is presented that cotton fibre adhesion and middle lamella formation are preceded by cutin dilution and accompanied by rhamnogalacturonan-I metabolism. Cotton fibres are single cell structures that early in development adhere to one another via the cotton fibre middle lamella (CFML) to form a tissue-like structure. The CFML is disassembled around the time of initial secondary wall deposition, leading to fibre detachment. Observations of CFML in the light microscope have suggested that the development of the middle lamella is accompanied by substantial cell-wall metabolism, but it has remained an open question as to which processes mediate adherence and which lead to detachment. The mechanism of adherence and detachment were investigated here using glyco-microarrays probed with monoclonal antibodies, transcript profiling, and observations of fibre auto-digestion. The results suggest that adherence is brought about by cutin dilution, while the presence of relevant enzyme activities and the dynamics of rhamnogalacturonan-I side-chain accumulation and disappearance suggest that both attachment and detachment are accompanied by rhamnogalacturonan-I metabolism.

Original language	English
Journal	Planta
Volume	249
Issue number	5
Pages (from-to)	1565–1581
ISSN	0032-0935
DOIs	https://doi.org/10.1007/s00425-019-03107-4
Publication status	Published - 1 May 2019

Keywords

Arabinofuranosidase
Cuticle
Post-genital fusion
Rhamnogalacturonan-I
Xyloglucan

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10.1007/s00425-019-03107-4

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title = "Metabolism of polysaccharides in dynamic middle lamellae during cotton fibre development",

abstract = "Main conclusion: Evidence is presented that cotton fibre adhesion and middle lamella formation are preceded by cutin dilution and accompanied by rhamnogalacturonan-I metabolism. Cotton fibres are single cell structures that early in development adhere to one another via the cotton fibre middle lamella (CFML) to form a tissue-like structure. The CFML is disassembled around the time of initial secondary wall deposition, leading to fibre detachment. Observations of CFML in the light microscope have suggested that the development of the middle lamella is accompanied by substantial cell-wall metabolism, but it has remained an open question as to which processes mediate adherence and which lead to detachment. The mechanism of adherence and detachment were investigated here using glyco-microarrays probed with monoclonal antibodies, transcript profiling, and observations of fibre auto-digestion. The results suggest that adherence is brought about by cutin dilution, while the presence of relevant enzyme activities and the dynamics of rhamnogalacturonan-I side-chain accumulation and disappearance suggest that both attachment and detachment are accompanied by rhamnogalacturonan-I metabolism.",

keywords = "Arabinofuranosidase, Cuticle, Post-genital fusion, Rhamnogalacturonan-I, Xyloglucan",

author = "Xiaoyuan Guo and Runavot, {Jean Luc} and St{\'e}phane Bourot and Frank Meulewaeter and Mercedes Hernandez-Gomez and Claire Holland and Jesper Harholt and Willats, {William G.T.} and Jozef Mravec and Paul Knox and Peter Ulvskov",

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doi = "10.1007/s00425-019-03107-4",

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T1 - Metabolism of polysaccharides in dynamic middle lamellae during cotton fibre development

AU - Guo, Xiaoyuan

AU - Runavot, Jean Luc

AU - Bourot, Stéphane

AU - Meulewaeter, Frank

AU - Hernandez-Gomez, Mercedes

AU - Holland, Claire

AU - Harholt, Jesper

AU - Willats, William G.T.

AU - Mravec, Jozef

AU - Knox, Paul

AU - Ulvskov, Peter

PY - 2019/5/1

Y1 - 2019/5/1

N2 - Main conclusion: Evidence is presented that cotton fibre adhesion and middle lamella formation are preceded by cutin dilution and accompanied by rhamnogalacturonan-I metabolism. Cotton fibres are single cell structures that early in development adhere to one another via the cotton fibre middle lamella (CFML) to form a tissue-like structure. The CFML is disassembled around the time of initial secondary wall deposition, leading to fibre detachment. Observations of CFML in the light microscope have suggested that the development of the middle lamella is accompanied by substantial cell-wall metabolism, but it has remained an open question as to which processes mediate adherence and which lead to detachment. The mechanism of adherence and detachment were investigated here using glyco-microarrays probed with monoclonal antibodies, transcript profiling, and observations of fibre auto-digestion. The results suggest that adherence is brought about by cutin dilution, while the presence of relevant enzyme activities and the dynamics of rhamnogalacturonan-I side-chain accumulation and disappearance suggest that both attachment and detachment are accompanied by rhamnogalacturonan-I metabolism.

AB - Main conclusion: Evidence is presented that cotton fibre adhesion and middle lamella formation are preceded by cutin dilution and accompanied by rhamnogalacturonan-I metabolism. Cotton fibres are single cell structures that early in development adhere to one another via the cotton fibre middle lamella (CFML) to form a tissue-like structure. The CFML is disassembled around the time of initial secondary wall deposition, leading to fibre detachment. Observations of CFML in the light microscope have suggested that the development of the middle lamella is accompanied by substantial cell-wall metabolism, but it has remained an open question as to which processes mediate adherence and which lead to detachment. The mechanism of adherence and detachment were investigated here using glyco-microarrays probed with monoclonal antibodies, transcript profiling, and observations of fibre auto-digestion. The results suggest that adherence is brought about by cutin dilution, while the presence of relevant enzyme activities and the dynamics of rhamnogalacturonan-I side-chain accumulation and disappearance suggest that both attachment and detachment are accompanied by rhamnogalacturonan-I metabolism.

KW - Arabinofuranosidase

KW - Cuticle

KW - Post-genital fusion

KW - Rhamnogalacturonan-I

KW - Xyloglucan

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DO - 10.1007/s00425-019-03107-4

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VL - 249

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Metabolism of polysaccharides in dynamic middle lamellae during cotton fibre development

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Keywords

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