Metabolism of polysaccharides in dynamic middle lamellae during cotton fibre development

TY - JOUR

T1 - Metabolism of polysaccharides in dynamic middle lamellae during cotton fibre development

AU - Guo, Xiaoyuan

AU - Runavot, Jean Luc

AU - Bourot, Stéphane

AU - Meulewaeter, Frank

AU - Hernandez-Gomez, Mercedes

AU - Holland, Claire

AU - Harholt, Jesper

AU - Willats, William G.T.

AU - Mravec, Jozef

AU - Knox, Paul

AU - Ulvskov, Peter

PY - 2019/5/1

Y1 - 2019/5/1

N2 - Main conclusion: Evidence is presented that cotton fibre adhesion and middle lamella formation are preceded by cutin dilution and accompanied by rhamnogalacturonan-I metabolism. Cotton fibres are single cell structures that early in development adhere to one another via the cotton fibre middle lamella (CFML) to form a tissue-like structure. The CFML is disassembled around the time of initial secondary wall deposition, leading to fibre detachment. Observations of CFML in the light microscope have suggested that the development of the middle lamella is accompanied by substantial cell-wall metabolism, but it has remained an open question as to which processes mediate adherence and which lead to detachment. The mechanism of adherence and detachment were investigated here using glyco-microarrays probed with monoclonal antibodies, transcript profiling, and observations of fibre auto-digestion. The results suggest that adherence is brought about by cutin dilution, while the presence of relevant enzyme activities and the dynamics of rhamnogalacturonan-I side-chain accumulation and disappearance suggest that both attachment and detachment are accompanied by rhamnogalacturonan-I metabolism.

AB - Main conclusion: Evidence is presented that cotton fibre adhesion and middle lamella formation are preceded by cutin dilution and accompanied by rhamnogalacturonan-I metabolism. Cotton fibres are single cell structures that early in development adhere to one another via the cotton fibre middle lamella (CFML) to form a tissue-like structure. The CFML is disassembled around the time of initial secondary wall deposition, leading to fibre detachment. Observations of CFML in the light microscope have suggested that the development of the middle lamella is accompanied by substantial cell-wall metabolism, but it has remained an open question as to which processes mediate adherence and which lead to detachment. The mechanism of adherence and detachment were investigated here using glyco-microarrays probed with monoclonal antibodies, transcript profiling, and observations of fibre auto-digestion. The results suggest that adherence is brought about by cutin dilution, while the presence of relevant enzyme activities and the dynamics of rhamnogalacturonan-I side-chain accumulation and disappearance suggest that both attachment and detachment are accompanied by rhamnogalacturonan-I metabolism.

KW - Arabinofuranosidase

KW - Cuticle

KW - Post-genital fusion

KW - Rhamnogalacturonan-I

KW - Xyloglucan

UR - http://www.scopus.com/inward/record.url?scp=85061293221&partnerID=8YFLogxK

U2 - 10.1007/s00425-019-03107-4

DO - 10.1007/s00425-019-03107-4

M3 - Journal article

C2 - 30737556

AN - SCOPUS:85061293221

SN - 0032-0943

VL - 249

SP - 1565

EP - 1581

JO - Planta Medica

JF - Planta Medica

IS - 5

ER -