Matrix assisted laser desorption/ionization mass spectrometry of enzymatically synthesized RNA up to 150 kDa

TY - JOUR

T1 - Matrix assisted laser desorption/ionization mass spectrometry of enzymatically synthesized RNA up to 150 kDa

AU - Kirpekar, F

AU - Nordhoff, E

AU - Kristiansen, K

AU - Roepstorff, P

AU - Lezius, A

AU - Hahner, S

AU - Karas, M

AU - Hillenkamp, F

N1 - Keywords: Base Sequence; DNA-Directed RNA Polymerases; Lasers; Mass Spectrometry; Molecular Sequence Data; Molecular Weight; Phosphoric Diester Hydrolases; RNA; Sequence Analysis, RNA

PY - 1994

Y1 - 1994

N2 - Enzymatically synthesized RNA samples (in vitro transcripts) were analysed by matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS). Spectra of RNA up to 150 kDA (461 nucleotides) are shown. Polymerase generated sample heterogeneity and its contribution to mass resolution are discussed. A time course exonuclease digest of a 55 nt in vitro transcript was analyzed to investigate the performance of MALDI-MS on complex mixtures. Based on these data, the analysis by MALDI-MS of DNA sequencing reactions, produced by the action of an RNA polymerase, is discussed.

AB - Enzymatically synthesized RNA samples (in vitro transcripts) were analysed by matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS). Spectra of RNA up to 150 kDA (461 nucleotides) are shown. Polymerase generated sample heterogeneity and its contribution to mass resolution are discussed. A time course exonuclease digest of a 55 nt in vitro transcript was analyzed to investigate the performance of MALDI-MS on complex mixtures. Based on these data, the analysis by MALDI-MS of DNA sequencing reactions, produced by the action of an RNA polymerase, is discussed.

M3 - Journal article

C2 - 7524029

SN - 0305-1048

VL - 22

SP - 3866

EP - 3870

JO - Nucleic Acids Research

JF - Nucleic Acids Research

IS - 19

ER -