@article{f2d055100f0b11de8478000ea68e967b,
title = "Matrix assisted laser desorption/ionization mass spectrometry of enzymatically synthesized RNA up to 150 kDa",
abstract = "Enzymatically synthesized RNA samples (in vitro transcripts) were analysed by matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS). Spectra of RNA up to 150 kDA (461 nucleotides) are shown. Polymerase generated sample heterogeneity and its contribution to mass resolution are discussed. A time course exonuclease digest of a 55 nt in vitro transcript was analyzed to investigate the performance of MALDI-MS on complex mixtures. Based on these data, the analysis by MALDI-MS of DNA sequencing reactions, produced by the action of an RNA polymerase, is discussed.",
author = "F Kirpekar and E Nordhoff and K Kristiansen and P Roepstorff and A Lezius and S Hahner and M Karas and F Hillenkamp",
note = "Keywords: Base Sequence; DNA-Directed RNA Polymerases; Lasers; Mass Spectrometry; Molecular Sequence Data; Molecular Weight; Phosphoric Diester Hydrolases; RNA; Sequence Analysis, RNA",
year = "1994",
language = "English",
volume = "22",
pages = "3866--70",
journal = "Nucleic Acids Research",
issn = "0305-1048",
publisher = "Oxford University Press",
number = "19",
}