Abstract
Three murine monoclonal antibodies (MAbs) F5-2, F5-4, and F5-16 defining three different epitopes on the major core protein p24 of the human immunodeficiency virus type 1 (HIV-1) were epitope mapped using a random fragment expression library representing the p17- and p24-encoding part of the gag open reading frame. F5-2 defined an epitope within amino acids (aa) 14-23 at the N-terminus of p24, and F5-4 defined an epitope within aa 153-174 in the C-terminus of p24. F5-16 did not recognize any of the fusion proteins produced by the expression library indicating that this MAb defines a true conformational epitope on p24. Since the N-terminus of p24 has been reported to be immunosilent in humans, 356 HIV-1 antibody-positive serum samples were tested for reactivity against the region of p24 defined by F5-2. More than one third of the samples recognized this region indicating that it is immunoreactive and, further, the presence of antibodies against this region was associated with a reduced CD4 cell count.
| Original language | English |
|---|---|
| Journal | AIDS Research and Human Retroviruses |
| Volume | 8 |
| Issue number | 10 |
| Pages (from-to) | 1789-94 |
| Number of pages | 6 |
| ISSN | 0889-2229 |
| DOIs | |
| Publication status | Published - Oct 1992 |
Keywords
- Amino Acid Sequence
- Animals
- Antibodies, Monoclonal
- B-Lymphocytes/immunology
- Base Sequence
- DNA, Viral/genetics
- Epitopes/genetics
- HIV Antibodies
- HIV Core Protein p24/genetics
- HIV-1/genetics
- Humans
- In Vitro Techniques
- Male
- Mice
- Molecular Sequence Data
