Mapping of linear B-cell epitopes on the major core protein p24 of human immunodeficiency virus type 1 (HIV-1)

TY - JOUR

T1 - Mapping of linear B-cell epitopes on the major core protein p24 of human immunodeficiency virus type 1 (HIV-1)

AU - Kusk, P

AU - Bugge, T H

AU - Lindhardt, B O

AU - Hulgaard, E F

AU - Holmbäck, K

PY - 1992/10

Y1 - 1992/10

N2 - Three murine monoclonal antibodies (MAbs) F5-2, F5-4, and F5-16 defining three different epitopes on the major core protein p24 of the human immunodeficiency virus type 1 (HIV-1) were epitope mapped using a random fragment expression library representing the p17- and p24-encoding part of the gag open reading frame. F5-2 defined an epitope within amino acids (aa) 14-23 at the N-terminus of p24, and F5-4 defined an epitope within aa 153-174 in the C-terminus of p24. F5-16 did not recognize any of the fusion proteins produced by the expression library indicating that this MAb defines a true conformational epitope on p24. Since the N-terminus of p24 has been reported to be immunosilent in humans, 356 HIV-1 antibody-positive serum samples were tested for reactivity against the region of p24 defined by F5-2. More than one third of the samples recognized this region indicating that it is immunoreactive and, further, the presence of antibodies against this region was associated with a reduced CD4 cell count.

AB - Three murine monoclonal antibodies (MAbs) F5-2, F5-4, and F5-16 defining three different epitopes on the major core protein p24 of the human immunodeficiency virus type 1 (HIV-1) were epitope mapped using a random fragment expression library representing the p17- and p24-encoding part of the gag open reading frame. F5-2 defined an epitope within amino acids (aa) 14-23 at the N-terminus of p24, and F5-4 defined an epitope within aa 153-174 in the C-terminus of p24. F5-16 did not recognize any of the fusion proteins produced by the expression library indicating that this MAb defines a true conformational epitope on p24. Since the N-terminus of p24 has been reported to be immunosilent in humans, 356 HIV-1 antibody-positive serum samples were tested for reactivity against the region of p24 defined by F5-2. More than one third of the samples recognized this region indicating that it is immunoreactive and, further, the presence of antibodies against this region was associated with a reduced CD4 cell count.

KW - Amino Acid Sequence

KW - Animals

KW - Antibodies, Monoclonal

KW - B-Lymphocytes/immunology

KW - Base Sequence

KW - DNA, Viral/genetics

KW - Epitopes/genetics

KW - HIV Antibodies

KW - HIV Core Protein p24/genetics

KW - HIV-1/genetics

KW - Humans

KW - In Vitro Techniques

KW - Male

KW - Mice

KW - Molecular Sequence Data

U2 - 10.1089/aid.1992.8.1789

DO - 10.1089/aid.1992.8.1789

M3 - Journal article

C2 - 1280956

SN - 0889-2229

VL - 8

SP - 1789

EP - 1794

JO - AIDS Research and Human Retroviruses

JF - AIDS Research and Human Retroviruses

IS - 10

ER -