Making bio-sense of toxicity: new developments in whole-cell biosencors

Søren Johannes Sørensen; Mette Burmølle; Lars Hestbjerg Hansen

doi:10.1016/j.copbio.2005.12.007

Making bio-sense of toxicity: new developments in whole-cell biosencors

Søren Johannes Sørensen, Mette Burmølle, Lars Hestbjerg Hansen

89 Citations (Scopus)

Abstract

Bacterial whole-cell biosensors are very useful for toxicity measurements of various samples. Semi-specific biosensors, containing fusions of stress-regulated promoters and reporter genes, have several advantages over the traditional, general biosensors that are based on constitutively expressed reporter genes. Furthermore, semi-specific biosensors are constantly being refined to lower their sensitivity and, in combination, are able to detect a wide range of toxic agents. However, the requirement for a positive response of these biosensors to toxicants can result in false-negative responses. The application of in situ inoculation and single-cell detection, combined with the introduction of new reporter genes and refined detection equipment, could lead to the extensive use of semi-specific, stress-responsive biosensors for toxicity estimations in the future.

Original language	English
Journal	Current Opinion in Biotechnology
Volume	17
Issue number	1
Pages (from-to)	11-16
ISSN	0958-1669
DOIs	https://doi.org/10.1016/j.copbio.2005.12.007
Publication status	Published - 2006

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abstract = "Bacterial whole-cell biosensors are very useful for toxicity measurements of various samples. Semi-specific biosensors, containing fusions of stress-regulated promoters and reporter genes, have several advantages over the traditional, general biosensors that are based on constitutively expressed reporter genes. Furthermore, semi-specific biosensors are constantly being refined to lower their sensitivity and, in combination, are able to detect a wide range of toxic agents. However, the requirement for a positive response of these biosensors to toxicants can result in false-negative responses. The application of in situ inoculation and single-cell detection, combined with the introduction of new reporter genes and refined detection equipment, could lead to the extensive use of semi-specific, stress-responsive biosensors for toxicity estimations in the future.",

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Making bio-sense of toxicity: new developments in whole-cell biosencors

Abstract

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