TY - JOUR
T1 - Making bio-sense of toxicity: new developments in whole-cell biosencors
AU - Sørensen, Søren Johannes
AU - Burmølle, Mette
AU - Hansen, Lars Hestbjerg
PY - 2006
Y1 - 2006
N2 - Bacterial whole-cell biosensors are very useful for toxicity measurements of various samples. Semi-specific biosensors, containing fusions of stress-regulated promoters and reporter genes, have several advantages over the traditional, general biosensors that are based on constitutively expressed reporter genes. Furthermore, semi-specific biosensors are constantly being refined to lower their sensitivity and, in combination, are able to detect a wide range of toxic agents. However, the requirement for a positive response of these biosensors to toxicants can result in false-negative responses. The application of in situ inoculation and single-cell detection, combined with the introduction of new reporter genes and refined detection equipment, could lead to the extensive use of semi-specific, stress-responsive biosensors for toxicity estimations in the future.
AB - Bacterial whole-cell biosensors are very useful for toxicity measurements of various samples. Semi-specific biosensors, containing fusions of stress-regulated promoters and reporter genes, have several advantages over the traditional, general biosensors that are based on constitutively expressed reporter genes. Furthermore, semi-specific biosensors are constantly being refined to lower their sensitivity and, in combination, are able to detect a wide range of toxic agents. However, the requirement for a positive response of these biosensors to toxicants can result in false-negative responses. The application of in situ inoculation and single-cell detection, combined with the introduction of new reporter genes and refined detection equipment, could lead to the extensive use of semi-specific, stress-responsive biosensors for toxicity estimations in the future.
U2 - 10.1016/j.copbio.2005.12.007
DO - 10.1016/j.copbio.2005.12.007
M3 - Journal article
C2 - 16376540
SN - 0958-1669
VL - 17
SP - 11
EP - 16
JO - Current Opinion in Biotechnology
JF - Current Opinion in Biotechnology
IS - 1
ER -