Lysozyme-magnesium aluminum silicate microparticles: Molecular interaction, bioactivity and release studies

Watchara Kanjanakawinkul; Natalie J. Medlicott; Thomas Rades; Satit Puttipipatkhachorn; Thaned Pongjanyakul

doi:10.1016/j.ijbiomac.2015.07.033

Lysozyme-magnesium aluminum silicate microparticles: Molecular interaction, bioactivity and release studies

Watchara Kanjanakawinkul, Natalie J. Medlicott, Thomas Rades, Satit Puttipipatkhachorn, Thaned Pongjanyakul

6 Citations (Scopus)

Abstract

The objectives of this study were to investigate the adsorption behavior of lysozyme (LSZ) onto magnesium aluminum silicate (MAS) at various pHs and to characterize the LSZ–MAS microparticles obtained from the molecular interaction between LSZ and MAS. The results showed that LSZ could be bound onto the MAS layers at different pHs, leading to the formation of LSZ–MAS microparticles. The higher preparation pH permitted greater adsorption affinity but a lower adsorption capacity of LSZ onto MAS. LSZ could interact with MAS via hydrogen bonds and electrostatic forces, resulting in the formation of intercalated nanocomposites. The particle size, %LSZ adsorbed, and LSZ release rate of LSZ–MAS microparticles increased when the LSZ–MAS ratio was increased. The secondary structure of LSZ bound onto the MAS layers in microparticles prepared at various pHs was altered compared with that of native LSZ. Moreover, the LSZ extracted from microparticles prepared at pH 4 showed an obvious change in the tertiary structure, leading to a decrease in the biological activity of the LSZ released. These findings suggested that LSZ can strongly interact with MAS to form microparticles that may potentially be used as delivery systems for sustained protein release.

Original language	English
Journal	International Journal of Biological Macromolecules
Volume	80
Pages (from-to)	651-658
Number of pages	8
ISSN	0141-8130
DOIs	https://doi.org/10.1016/j.ijbiomac.2015.07.033
Publication status	Published - 1 Sept 2015

Keywords

Lysozyme
Magnesium aluminum silicate
Microparticles
Protein conformation
Bioactivity
Protein release

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10.1016/j.ijbiomac.2015.07.033

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@article{3b0af36245d94536b8ad6acd57a5434a,

title = "Lysozyme-magnesium aluminum silicate microparticles: Molecular interaction, bioactivity and release studies",

abstract = "The objectives of this study were to investigate the adsorption behavior of lysozyme (LSZ) onto magnesium aluminum silicate (MAS) at various pHs and to characterize the LSZ–MAS microparticles obtained from the molecular interaction between LSZ and MAS. The results showed that LSZ could be bound onto the MAS layers at different pHs, leading to the formation of LSZ–MAS microparticles. The higher preparation pH permitted greater adsorption affinity but a lower adsorption capacity of LSZ onto MAS. LSZ could interact with MAS via hydrogen bonds and electrostatic forces, resulting in the formation of intercalated nanocomposites. The particle size, %LSZ adsorbed, and LSZ release rate of LSZ–MAS microparticles increased when the LSZ–MAS ratio was increased. The secondary structure of LSZ bound onto the MAS layers in microparticles prepared at various pHs was altered compared with that of native LSZ. Moreover, the LSZ extracted from microparticles prepared at pH 4 showed an obvious change in the tertiary structure, leading to a decrease in the biological activity of the LSZ released. These findings suggested that LSZ can strongly interact with MAS to form microparticles that may potentially be used as delivery systems for sustained protein release.",

keywords = "Lysozyme, Magnesium aluminum silicate, Microparticles, Protein conformation, Bioactivity, Protein release",

author = "Watchara Kanjanakawinkul and Medlicott, {Natalie J.} and Thomas Rades and Satit Puttipipatkhachorn and Thaned Pongjanyakul",

year = "2015",

month = sep,

day = "1",

doi = "10.1016/j.ijbiomac.2015.07.033",

language = "English",

volume = "80",

pages = "651--658",

journal = "International Journal of Biological Macromolecules",

issn = "0141-8130",

publisher = "Elsevier",

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TY - JOUR

T1 - Lysozyme-magnesium aluminum silicate microparticles: Molecular interaction, bioactivity and release studies

AU - Kanjanakawinkul, Watchara

AU - Medlicott, Natalie J.

AU - Rades, Thomas

AU - Puttipipatkhachorn, Satit

AU - Pongjanyakul, Thaned

PY - 2015/9/1

Y1 - 2015/9/1

N2 - The objectives of this study were to investigate the adsorption behavior of lysozyme (LSZ) onto magnesium aluminum silicate (MAS) at various pHs and to characterize the LSZ–MAS microparticles obtained from the molecular interaction between LSZ and MAS. The results showed that LSZ could be bound onto the MAS layers at different pHs, leading to the formation of LSZ–MAS microparticles. The higher preparation pH permitted greater adsorption affinity but a lower adsorption capacity of LSZ onto MAS. LSZ could interact with MAS via hydrogen bonds and electrostatic forces, resulting in the formation of intercalated nanocomposites. The particle size, %LSZ adsorbed, and LSZ release rate of LSZ–MAS microparticles increased when the LSZ–MAS ratio was increased. The secondary structure of LSZ bound onto the MAS layers in microparticles prepared at various pHs was altered compared with that of native LSZ. Moreover, the LSZ extracted from microparticles prepared at pH 4 showed an obvious change in the tertiary structure, leading to a decrease in the biological activity of the LSZ released. These findings suggested that LSZ can strongly interact with MAS to form microparticles that may potentially be used as delivery systems for sustained protein release.

AB - The objectives of this study were to investigate the adsorption behavior of lysozyme (LSZ) onto magnesium aluminum silicate (MAS) at various pHs and to characterize the LSZ–MAS microparticles obtained from the molecular interaction between LSZ and MAS. The results showed that LSZ could be bound onto the MAS layers at different pHs, leading to the formation of LSZ–MAS microparticles. The higher preparation pH permitted greater adsorption affinity but a lower adsorption capacity of LSZ onto MAS. LSZ could interact with MAS via hydrogen bonds and electrostatic forces, resulting in the formation of intercalated nanocomposites. The particle size, %LSZ adsorbed, and LSZ release rate of LSZ–MAS microparticles increased when the LSZ–MAS ratio was increased. The secondary structure of LSZ bound onto the MAS layers in microparticles prepared at various pHs was altered compared with that of native LSZ. Moreover, the LSZ extracted from microparticles prepared at pH 4 showed an obvious change in the tertiary structure, leading to a decrease in the biological activity of the LSZ released. These findings suggested that LSZ can strongly interact with MAS to form microparticles that may potentially be used as delivery systems for sustained protein release.

KW - Lysozyme

KW - Magnesium aluminum silicate

KW - Microparticles

KW - Protein conformation

KW - Bioactivity

KW - Protein release

U2 - 10.1016/j.ijbiomac.2015.07.033

DO - 10.1016/j.ijbiomac.2015.07.033

M3 - Journal article

C2 - 26193680

SN - 0141-8130

VL - 80

SP - 651

EP - 658

JO - International Journal of Biological Macromolecules

JF - International Journal of Biological Macromolecules

ER -

Lysozyme-magnesium aluminum silicate microparticles: Molecular interaction, bioactivity and release studies

Abstract

Keywords

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