TY - JOUR
T1 - Lysozyme-magnesium aluminum silicate microparticles: Molecular interaction, bioactivity and release studies
AU - Kanjanakawinkul, Watchara
AU - Medlicott, Natalie J.
AU - Rades, Thomas
AU - Puttipipatkhachorn, Satit
AU - Pongjanyakul, Thaned
PY - 2015/9/1
Y1 - 2015/9/1
N2 - The objectives of this study were to investigate the adsorption behavior of lysozyme (LSZ) onto magnesium aluminum silicate (MAS) at various pHs and to characterize the LSZ–MAS microparticles obtained from the molecular interaction between LSZ and MAS. The results showed that LSZ could be bound onto the MAS layers at different pHs, leading to the formation of LSZ–MAS microparticles. The higher preparation pH permitted greater adsorption affinity but a lower adsorption capacity of LSZ onto MAS. LSZ could interact with MAS via hydrogen bonds and electrostatic forces, resulting in the formation of intercalated nanocomposites. The particle size, %LSZ adsorbed, and LSZ release rate of LSZ–MAS microparticles increased when the LSZ–MAS ratio was increased. The secondary structure of LSZ bound onto the MAS layers in microparticles prepared at various pHs was altered compared with that of native LSZ. Moreover, the LSZ extracted from microparticles prepared at pH 4 showed an obvious change in the tertiary structure, leading to a decrease in the biological activity of the LSZ released. These findings suggested that LSZ can strongly interact with MAS to form microparticles that may potentially be used as delivery systems for sustained protein release.
AB - The objectives of this study were to investigate the adsorption behavior of lysozyme (LSZ) onto magnesium aluminum silicate (MAS) at various pHs and to characterize the LSZ–MAS microparticles obtained from the molecular interaction between LSZ and MAS. The results showed that LSZ could be bound onto the MAS layers at different pHs, leading to the formation of LSZ–MAS microparticles. The higher preparation pH permitted greater adsorption affinity but a lower adsorption capacity of LSZ onto MAS. LSZ could interact with MAS via hydrogen bonds and electrostatic forces, resulting in the formation of intercalated nanocomposites. The particle size, %LSZ adsorbed, and LSZ release rate of LSZ–MAS microparticles increased when the LSZ–MAS ratio was increased. The secondary structure of LSZ bound onto the MAS layers in microparticles prepared at various pHs was altered compared with that of native LSZ. Moreover, the LSZ extracted from microparticles prepared at pH 4 showed an obvious change in the tertiary structure, leading to a decrease in the biological activity of the LSZ released. These findings suggested that LSZ can strongly interact with MAS to form microparticles that may potentially be used as delivery systems for sustained protein release.
KW - Lysozyme
KW - Magnesium aluminum silicate
KW - Microparticles
KW - Protein conformation
KW - Bioactivity
KW - Protein release
U2 - 10.1016/j.ijbiomac.2015.07.033
DO - 10.1016/j.ijbiomac.2015.07.033
M3 - Journal article
C2 - 26193680
SN - 0141-8130
VL - 80
SP - 651
EP - 658
JO - International Journal of Biological Macromolecules
JF - International Journal of Biological Macromolecules
ER -