Luminescence lifetime imaging of chemical sensors – a comparison between time-domain and frequency-domain based camera systems

Klaus Koren; Maria Mosshammer; Vincent Valentin Scholz; Sergey M. Borisov; Gerhard Holst; Michael Kühl

doi:10.1021/acs.analchem.8b05869

Luminescence lifetime imaging of chemical sensors – a comparison between time-domain and frequency-domain based camera systems

Klaus Koren, Maria Mosshammer, Vincent Valentin Scholz, Sergey M. Borisov, Gerhard Holst, Michael Kühl

Marine Biology

16 Citations (Scopus)

Abstract

Luminescence lifetime based imaging is still the most reliable method for generating chemical images using chemical sensor technology. However, only few commercial systems are available that enable imaging lifetimes within the relevant nanosecond to microsecond range. In this technical note we compare the performance of an older time-domain (TD) based camera system with a frequency-domain (FD) based camera system regarding their measuring characteristics and applicability for O ₂ and pH imaging in environmental samples and with different indicator dye systems emitting in the visible and near-infrared part of the spectrum. We conclude that the newly introduced FD imaging system delivers comparable if not better results than its predecessor, now enabling robust and simple chemical imaging based on FD luminescence lifetime measurements.

Original language	English
Journal	Analytical Chemistry
Volume	91
Issue number	5
Pages (from-to)	3233-3238
ISSN	0003-2700
DOIs	https://doi.org/10.1021/acs.analchem.8b05869
Publication status	Published - 5 Mar 2019

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abstract = " Luminescence lifetime based imaging is still the most reliable method for generating chemical images using chemical sensor technology. However, only few commercial systems are available that enable imaging lifetimes within the relevant nanosecond to microsecond range. In this technical note we compare the performance of an older time-domain (TD) based camera system with a frequency-domain (FD) based camera system regarding their measuring characteristics and applicability for O 2 and pH imaging in environmental samples and with different indicator dye systems emitting in the visible and near-infrared part of the spectrum. We conclude that the newly introduced FD imaging system delivers comparable if not better results than its predecessor, now enabling robust and simple chemical imaging based on FD luminescence lifetime measurements. ",

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AU - Scholz, Vincent Valentin

AU - Borisov, Sergey M.

AU - Holst, Gerhard

AU - Kühl, Michael

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AB - Luminescence lifetime based imaging is still the most reliable method for generating chemical images using chemical sensor technology. However, only few commercial systems are available that enable imaging lifetimes within the relevant nanosecond to microsecond range. In this technical note we compare the performance of an older time-domain (TD) based camera system with a frequency-domain (FD) based camera system regarding their measuring characteristics and applicability for O 2 and pH imaging in environmental samples and with different indicator dye systems emitting in the visible and near-infrared part of the spectrum. We conclude that the newly introduced FD imaging system delivers comparable if not better results than its predecessor, now enabling robust and simple chemical imaging based on FD luminescence lifetime measurements.

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Luminescence lifetime imaging of chemical sensors – a comparison between time-domain and frequency-domain based camera systems

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