Luminescence lifetime imaging of chemical sensors – a comparison between time-domain and frequency-domain based camera systems

Klaus Koren; Maria Mosshammer; Vincent Valentin Scholz; Sergey M. Borisov; Gerhard Holst; Michael Kühl

doi:10.1021/acs.analchem.8b05869

Luminescence lifetime imaging of chemical sensors – a comparison between time-domain and frequency-domain based camera systems

Klaus Koren, Maria Mosshammer, Vincent Valentin Scholz, Sergey M. Borisov, Gerhard Holst, Michael Kühl

Marine Biological Section

16 Citationer (Scopus)

Abstract

Luminescence lifetime based imaging is still the most reliable method for generating chemical images using chemical sensor technology. However, only few commercial systems are available that enable imaging lifetimes within the relevant nanosecond to microsecond range. In this technical note we compare the performance of an older time-domain (TD) based camera system with a frequency-domain (FD) based camera system regarding their measuring characteristics and applicability for O ₂ and pH imaging in environmental samples and with different indicator dye systems emitting in the visible and near-infrared part of the spectrum. We conclude that the newly introduced FD imaging system delivers comparable if not better results than its predecessor, now enabling robust and simple chemical imaging based on FD luminescence lifetime measurements.

Originalsprog	Engelsk
Tidsskrift	Analytical Chemistry
Vol/bind	91
Udgave nummer	5
Sider (fra-til)	3233-3238
ISSN	0003-2700
DOI	https://doi.org/10.1021/acs.analchem.8b05869
Status	Udgivet - 5 mar. 2019

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10.1021/acs.analchem.8b05869

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abstract = " Luminescence lifetime based imaging is still the most reliable method for generating chemical images using chemical sensor technology. However, only few commercial systems are available that enable imaging lifetimes within the relevant nanosecond to microsecond range. In this technical note we compare the performance of an older time-domain (TD) based camera system with a frequency-domain (FD) based camera system regarding their measuring characteristics and applicability for O 2 and pH imaging in environmental samples and with different indicator dye systems emitting in the visible and near-infrared part of the spectrum. We conclude that the newly introduced FD imaging system delivers comparable if not better results than its predecessor, now enabling robust and simple chemical imaging based on FD luminescence lifetime measurements. ",

author = "Klaus Koren and Maria Mosshammer and Scholz, {Vincent Valentin} and Borisov, {Sergey M.} and Gerhard Holst and Michael K{\"u}hl",

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AU - Koren, Klaus

AU - Mosshammer, Maria

AU - Scholz, Vincent Valentin

AU - Borisov, Sergey M.

AU - Holst, Gerhard

AU - Kühl, Michael

PY - 2019/3/5

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N2 - Luminescence lifetime based imaging is still the most reliable method for generating chemical images using chemical sensor technology. However, only few commercial systems are available that enable imaging lifetimes within the relevant nanosecond to microsecond range. In this technical note we compare the performance of an older time-domain (TD) based camera system with a frequency-domain (FD) based camera system regarding their measuring characteristics and applicability for O 2 and pH imaging in environmental samples and with different indicator dye systems emitting in the visible and near-infrared part of the spectrum. We conclude that the newly introduced FD imaging system delivers comparable if not better results than its predecessor, now enabling robust and simple chemical imaging based on FD luminescence lifetime measurements.

AB - Luminescence lifetime based imaging is still the most reliable method for generating chemical images using chemical sensor technology. However, only few commercial systems are available that enable imaging lifetimes within the relevant nanosecond to microsecond range. In this technical note we compare the performance of an older time-domain (TD) based camera system with a frequency-domain (FD) based camera system regarding their measuring characteristics and applicability for O 2 and pH imaging in environmental samples and with different indicator dye systems emitting in the visible and near-infrared part of the spectrum. We conclude that the newly introduced FD imaging system delivers comparable if not better results than its predecessor, now enabling robust and simple chemical imaging based on FD luminescence lifetime measurements.

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JO - Analytical Chemistry

JF - Analytical Chemistry

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Luminescence lifetime imaging of chemical sensors – a comparison between time-domain and frequency-domain based camera systems

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