Label-free DNA electrochemical sensor based on a PNA-functionalized conductive polymer.

S Reisberg; L A Dang; Q A Nguyen; B Piro; V Noel; Peter E. Nielsen; L A Le; M C Pham

doi:10.1016/j.talanta.2008.02.044

Label-free DNA electrochemical sensor based on a PNA-functionalized conductive polymer.

S Reisberg, L A Dang, Q A Nguyen, B Piro, V Noel, Peter E. Nielsen, L A Le, M C Pham

Department of Cellular and Molecular Medicine

50 Citations (Scopus)

Abstract

An electrochemical hybridization biosensor based on peptide nucleic acid (PNA) probe is presented. PNA were attached covalently onto a quinone-based electroactive polymer. Changes in flexibility of the PNA probe strand upon hybridization generates electrochemical changes at the polymer-solution interface. A reagentless and direct electrochemical detection was obtained by detection of the electrochemical changes using square wave voltammetry (SWV). An increase in the peak current of quinone was observed upon hybridization of probe on the target, whereas no change is observed with non-complementary sequence. In addition, the biosensor is highly selective to effectively discriminate a single mismatch on the target sequence. The sensitivity is also presented and discussed.

Original language	English
Journal	Talanta: The International Journal of Pure and Applied Analytical Chemistry
Volume	76
Issue number	1
Pages (from-to)	206-10
Number of pages	4
ISSN	0039-9140
DOIs	https://doi.org/10.1016/j.talanta.2008.02.044
Publication status	Published - 2008

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10.1016/j.talanta.2008.02.044

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title = "Label-free DNA electrochemical sensor based on a PNA-functionalized conductive polymer.",

abstract = "An electrochemical hybridization biosensor based on peptide nucleic acid (PNA) probe is presented. PNA were attached covalently onto a quinone-based electroactive polymer. Changes in flexibility of the PNA probe strand upon hybridization generates electrochemical changes at the polymer-solution interface. A reagentless and direct electrochemical detection was obtained by detection of the electrochemical changes using square wave voltammetry (SWV). An increase in the peak current of quinone was observed upon hybridization of probe on the target, whereas no change is observed with non-complementary sequence. In addition, the biosensor is highly selective to effectively discriminate a single mismatch on the target sequence. The sensitivity is also presented and discussed.",

author = "S Reisberg and Dang, {L A} and Nguyen, {Q A} and B Piro and V Noel and Nielsen, {Peter E.} and Le, {L A} and Pham, {M C}",

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language = "English",

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T1 - Label-free DNA electrochemical sensor based on a PNA-functionalized conductive polymer.

AU - Reisberg, S

AU - Dang, L A

AU - Nguyen, Q A

AU - Piro, B

AU - Noel, V

AU - Nielsen, Peter E.

AU - Le, L A

AU - Pham, M C

PY - 2008

Y1 - 2008

N2 - An electrochemical hybridization biosensor based on peptide nucleic acid (PNA) probe is presented. PNA were attached covalently onto a quinone-based electroactive polymer. Changes in flexibility of the PNA probe strand upon hybridization generates electrochemical changes at the polymer-solution interface. A reagentless and direct electrochemical detection was obtained by detection of the electrochemical changes using square wave voltammetry (SWV). An increase in the peak current of quinone was observed upon hybridization of probe on the target, whereas no change is observed with non-complementary sequence. In addition, the biosensor is highly selective to effectively discriminate a single mismatch on the target sequence. The sensitivity is also presented and discussed.

AB - An electrochemical hybridization biosensor based on peptide nucleic acid (PNA) probe is presented. PNA were attached covalently onto a quinone-based electroactive polymer. Changes in flexibility of the PNA probe strand upon hybridization generates electrochemical changes at the polymer-solution interface. A reagentless and direct electrochemical detection was obtained by detection of the electrochemical changes using square wave voltammetry (SWV). An increase in the peak current of quinone was observed upon hybridization of probe on the target, whereas no change is observed with non-complementary sequence. In addition, the biosensor is highly selective to effectively discriminate a single mismatch on the target sequence. The sensitivity is also presented and discussed.

U2 - 10.1016/j.talanta.2008.02.044

DO - 10.1016/j.talanta.2008.02.044

M3 - Journal article

C2 - 18585264

SN - 0039-9140

VL - 76

SP - 206

EP - 210

JO - Talanta

JF - Talanta

IS - 1

ER -

Label-free DNA electrochemical sensor based on a PNA-functionalized conductive polymer.

Abstract

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