Label-free and reagentless electrochemical detection of PCR fragments using self-assembled quinone derivative monolayer: Application to Mycobacterium tuberculosis

TY - JOUR

T1 - Label-free and reagentless electrochemical detection of PCR fragments using self-assembled quinone derivative monolayer: Application to Mycobacterium tuberculosis

AU - Zhang, Q D

AU - March, G

AU - Noel, V

AU - Piro, B

AU - Reisberg, S

AU - Tran, L D

AU - Hai, L V

AU - Abadia, E

AU - Nielsen, P E

AU - Sola, C

AU - Pham, M C

N1 - Copyright © 2011 Elsevier B.V. All rights reserved.

PY - 2012/2/15

Y1 - 2012/2/15

N2 - We report a signal-on, label-free and reagentless electrochemical DNA biosensor, based on a mixed self-assembled monolayer of thiolated hydroxynaphthoquinone and thiolated oligonucleotide. Electrochemical changes resulting from hybridization were evidenced with oligonucleotide targets (as models), as well as with polymerase chain reaction (PCR) products related to different lineages of Mycobacterium tuberculosis strains. With pure oligonucleotides, this system achieves high sensitivity (~300pM of DNA target, i.e. 30fmol in a 100µL sample) and excellent selectivity, allowing to detect a single mismatch on a sequence of 20 bases. With PCR products, current changes are specific to the bacterial strain from which the PCR fragment is produced. In addition, the sensor response is of the signal-on type, giving a positive signal change upon hybridization, and therefore does not suffer from false positive responses due to non-specific adsorption of DNA.

AB - We report a signal-on, label-free and reagentless electrochemical DNA biosensor, based on a mixed self-assembled monolayer of thiolated hydroxynaphthoquinone and thiolated oligonucleotide. Electrochemical changes resulting from hybridization were evidenced with oligonucleotide targets (as models), as well as with polymerase chain reaction (PCR) products related to different lineages of Mycobacterium tuberculosis strains. With pure oligonucleotides, this system achieves high sensitivity (~300pM of DNA target, i.e. 30fmol in a 100µL sample) and excellent selectivity, allowing to detect a single mismatch on a sequence of 20 bases. With PCR products, current changes are specific to the bacterial strain from which the PCR fragment is produced. In addition, the sensor response is of the signal-on type, giving a positive signal change upon hybridization, and therefore does not suffer from false positive responses due to non-specific adsorption of DNA.

U2 - 10.1016/j.bios.2011.11.048

DO - 10.1016/j.bios.2011.11.048

M3 - Journal article

C2 - 22186165

SN - 2155-6210

VL - 32

SP - 163

EP - 168

JO - Journal of Biosensors & Bioelectronics

JF - Journal of Biosensors & Bioelectronics

IS - 1

ER -