KCNQ1 channels sense small changes in cell volume.

Morten Grunnet; Thomas Jespersen; Nanna MacAulay; Nanna K Jørgensen; Nicole Schmitt; Olaf Pongs; Søren-Peter Olesen; Dan Arne Klærke

doi:10.1113/jphysiol.2003.038455

KCNQ1 channels sense small changes in cell volume.

Morten Grunnet, Thomas Jespersen, Nanna MacAulay, Nanna K Jørgensen, Nicole Schmitt, Olaf Pongs, Søren-Peter Olesen, Dan Arne Klærke

76 Citations (Scopus)

Abstract

Many important physiological processes involve changes in cell volume, e.g. the transport of salt and water in epithelial cells and the contraction of cardiomyocytes. In this study, we show that voltage-gated KCNQ1 channels, which are strongly expressed in epithelial cells or cardiomyocytes, and KCNQ4 channels, expressed in hair cells and the auditory tract, are tightly regulated by small cell volume changes when co-expressed with aquaporin 1 water-channels (AQP1) in Xenopus oocytes. The KCNQ1 and KCNQ4 current amplitudes precisely reflect the volume of the oocytes. By contrast, the related KCNQ2 and KCNQ3 channels, which are prominently expressed in neurons, are insensitive to cell volume changes. The sensitivity of the KCNQ1 and KCNQ4 channels to cell volume changes is independent of the presence of the auxiliary KCNE1-3 subunits, although modulated by KCNE1 in the case of KCNQ1. Incubation of the oocytes in cytochalasin D and experiments with truncated KCNQ1 channels suggest that KCNQ1 channels sense cell volume changes through interactions between the cytoskeleton and the N-terminus of the channel protein. From our results we propose that KCNQ1 and KCNQ4 channels play an important role in cell volume control, e.g. during transepithelial transport of salt and water.
Udgivelsesdato: 2003-Jun-1

Original language	English
Journal	Journal of Physiology
Volume	549
Issue number	Pt 2
Pages (from-to)	419-27
Number of pages	8
ISSN	0022-3751
DOIs	https://doi.org/10.1113/jphysiol.2003.038455
Publication status	Published - 2003

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10.1113/jphysiol.2003.038455

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@article{b7eda670e92111dcbee902004c4f4f50,

title = "KCNQ1 channels sense small changes in cell volume.",

abstract = "Many important physiological processes involve changes in cell volume, e.g. the transport of salt and water in epithelial cells and the contraction of cardiomyocytes. In this study, we show that voltage-gated KCNQ1 channels, which are strongly expressed in epithelial cells or cardiomyocytes, and KCNQ4 channels, expressed in hair cells and the auditory tract, are tightly regulated by small cell volume changes when co-expressed with aquaporin 1 water-channels (AQP1) in Xenopus oocytes. The KCNQ1 and KCNQ4 current amplitudes precisely reflect the volume of the oocytes. By contrast, the related KCNQ2 and KCNQ3 channels, which are prominently expressed in neurons, are insensitive to cell volume changes. The sensitivity of the KCNQ1 and KCNQ4 channels to cell volume changes is independent of the presence of the auxiliary KCNE1-3 subunits, although modulated by KCNE1 in the case of KCNQ1. Incubation of the oocytes in cytochalasin D and experiments with truncated KCNQ1 channels suggest that KCNQ1 channels sense cell volume changes through interactions between the cytoskeleton and the N-terminus of the channel protein. From our results we propose that KCNQ1 and KCNQ4 channels play an important role in cell volume control, e.g. during transepithelial transport of salt and water. Udgivelsesdato: 2003-Jun-1",

author = "Morten Grunnet and Thomas Jespersen and Nanna MacAulay and J{\o}rgensen, {Nanna K} and Nicole Schmitt and Olaf Pongs and S{\o}ren-Peter Olesen and Kl{\ae}rke, {Dan Arne}",

note = "Keywords: Animals; Aquaporin 1; Aquaporins; Cell Size; Electrophysiology; KCNQ Potassium Channels; KCNQ1 Potassium Channel; Oocytes; Potassium Channels; Potassium Channels, Voltage-Gated; Xenopus laevis",

year = "2003",

doi = "10.1113/jphysiol.2003.038455",

language = "English",

volume = "549",

pages = "419--27",

journal = "The Journal of Physiology",

issn = "0022-3751",

publisher = "Wiley-Blackwell",

number = "Pt 2",

}

TY - JOUR

T1 - KCNQ1 channels sense small changes in cell volume.

AU - Grunnet, Morten

AU - Jespersen, Thomas

AU - MacAulay, Nanna

AU - Jørgensen, Nanna K

AU - Schmitt, Nicole

AU - Pongs, Olaf

AU - Olesen, Søren-Peter

AU - Klærke, Dan Arne

N1 - Keywords: Animals; Aquaporin 1; Aquaporins; Cell Size; Electrophysiology; KCNQ Potassium Channels; KCNQ1 Potassium Channel; Oocytes; Potassium Channels; Potassium Channels, Voltage-Gated; Xenopus laevis

PY - 2003

Y1 - 2003

N2 - Many important physiological processes involve changes in cell volume, e.g. the transport of salt and water in epithelial cells and the contraction of cardiomyocytes. In this study, we show that voltage-gated KCNQ1 channels, which are strongly expressed in epithelial cells or cardiomyocytes, and KCNQ4 channels, expressed in hair cells and the auditory tract, are tightly regulated by small cell volume changes when co-expressed with aquaporin 1 water-channels (AQP1) in Xenopus oocytes. The KCNQ1 and KCNQ4 current amplitudes precisely reflect the volume of the oocytes. By contrast, the related KCNQ2 and KCNQ3 channels, which are prominently expressed in neurons, are insensitive to cell volume changes. The sensitivity of the KCNQ1 and KCNQ4 channels to cell volume changes is independent of the presence of the auxiliary KCNE1-3 subunits, although modulated by KCNE1 in the case of KCNQ1. Incubation of the oocytes in cytochalasin D and experiments with truncated KCNQ1 channels suggest that KCNQ1 channels sense cell volume changes through interactions between the cytoskeleton and the N-terminus of the channel protein. From our results we propose that KCNQ1 and KCNQ4 channels play an important role in cell volume control, e.g. during transepithelial transport of salt and water. Udgivelsesdato: 2003-Jun-1

AB - Many important physiological processes involve changes in cell volume, e.g. the transport of salt and water in epithelial cells and the contraction of cardiomyocytes. In this study, we show that voltage-gated KCNQ1 channels, which are strongly expressed in epithelial cells or cardiomyocytes, and KCNQ4 channels, expressed in hair cells and the auditory tract, are tightly regulated by small cell volume changes when co-expressed with aquaporin 1 water-channels (AQP1) in Xenopus oocytes. The KCNQ1 and KCNQ4 current amplitudes precisely reflect the volume of the oocytes. By contrast, the related KCNQ2 and KCNQ3 channels, which are prominently expressed in neurons, are insensitive to cell volume changes. The sensitivity of the KCNQ1 and KCNQ4 channels to cell volume changes is independent of the presence of the auxiliary KCNE1-3 subunits, although modulated by KCNE1 in the case of KCNQ1. Incubation of the oocytes in cytochalasin D and experiments with truncated KCNQ1 channels suggest that KCNQ1 channels sense cell volume changes through interactions between the cytoskeleton and the N-terminus of the channel protein. From our results we propose that KCNQ1 and KCNQ4 channels play an important role in cell volume control, e.g. during transepithelial transport of salt and water. Udgivelsesdato: 2003-Jun-1

U2 - 10.1113/jphysiol.2003.038455

DO - 10.1113/jphysiol.2003.038455

M3 - Journal article

C2 - 12702742

SN - 0022-3751

VL - 549

SP - 419

EP - 427

JO - The Journal of Physiology

JF - The Journal of Physiology

IS - Pt 2

ER -