Isolation of monodisperse nanodisc-reconstituted membrane proteins using free flow electrophoresis

14 Citations (Scopus)

Abstract

Free flow electrophoresis is used for rapid and high-recovery isolation of homogeneous preparations of functionally active membrane proteins inserted into nanodiscs. The approach enables isolation of integral and membrane anchored proteins and is also applicable following introduction of, e.g., fluorescent tags. Preparative separation of membrane protein loaded nanodiscs from empty nanodiscs and protein aggregates results in monodisperse nanodisc preparations ideal for structural and functional characterization using biophysical methods.
Original languageEnglish
JournalAnalytical Chemistry
Volume85
Issue number7
Pages (from-to)3497-3500
Number of pages4
ISSN0003-2700
DOIs
Publication statusPublished - 2 Apr 2013

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